| 匹多莫德增强弓形虫GRA1蛋白免疫效果的动物实验评价 |
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| 引用本文： | 谢荣华.匹多莫德增强弓形虫GRA1蛋白免疫效果的动物实验评价[J].中国免疫学杂志,2011,27(8):748-750,756. |
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| 作者姓名： | 谢荣华 |
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| 作者单位： | 湖南环境生物职业技术学院,衡阳,421005 |
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| 基金项目： | 湖南省卫生厅资助项目，湖南环境生物职业技术学院院长基金(No.Z07-09)项目 |
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| 摘 要： | 目的:探讨匹多莫德增强弓形虫GRA1蛋白刺激机体产生免疫应答和保护免疫的效果。方法:匹多莫德和毕赤酵母菌表达的弓形虫GRA1蛋白混合皮下注射免疫小鼠,以PBS作对照,检测其细胞免疫和体液免疫,并观察其受到弓形虫攻击感染后的生存情况。结果:匹多莫德能辅助GRA1蛋白刺激宿主产生较高的细胞免疫和体液免疫,并能提供较好的免疫保护力。GRA1蛋白加匹多莫德组、GRA1蛋白加弗氏完全佐剂组小鼠IFNγ-、特异性IgG显著高于对照组(P<0.01)和GRA1蛋白组(P<0.01);各免疫组CD8+T细胞数量显著高于对照组(P<0.01);GRA1蛋白加匹多莫德组CD4+T细胞数量(P<0.01)、CD4+/CD8+比值显著高于其它免疫组(P<0.05)。各免疫组T细胞增殖活性与PBS对照组比较明显增强(P<0.01),GRA1蛋白加匹多莫德组T细胞增殖活性最明显。小鼠攻击试验表明,GRA1蛋白加匹多莫德组和GRA1蛋白加弗氏完全佐剂组小鼠存活时间明显长于对照组和GRA1蛋白组。结论:匹多莫德与弗氏佐剂具有相似增强免疫应答和提高GRA1蛋白抗原的免疫原性作用,可望用于弓形虫亚单位疫苗的研制。
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| 关 键 词： | 弓形虫 匹多莫德 毕赤酵母菌 GRA1蛋白 免疫效果 |
Evaluation of immune enhancement by GRA1 protein of Toxoplasma gondii by Pidotimod in mice |
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| XIE Rong-Hua.Evaluation of immune enhancement by GRA1 protein of Toxoplasma gondii by Pidotimod in mice[J].Chinese Journal of Immunology,2011,27(8):748-750,756. |
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| Authors: | XIE Rong-Hua |
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| Institution: | XIE Rong-Hua.Enviroment Biology of Technology College,Hengyang 421005,China |
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| Abstract: | Objective:To observe the immune response and protective immunity in mice induce by GRA1 protein expressed in pichia with pidotimod.Methods:Mice were immunzed with GRA1 protein and pidotimod,and those mice receiving PBS were used as controls,then the immune response and the survival time of mouse attacked by Toxoplasma gondii were observed.Results:The highest immune respose was induced by GRA1 protein combined with pidotimod.This vaccine and adjuvand can offer good protective immunity.After the immunization,... |
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| Keywords: | Toxoplasma gondii Pidotimod Pichia pastoris GRA1 protein Immunity effect |
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