严重感染性休克患者血中分离的一株Klebsiella variicola菌株的鉴定

目的 对一株临床分离的Klebsiella variicola疑似菌株进行鉴定.方法 扩增该临床分离菌株的rpoB、gyrA、mdh、infB、phoE和nifH六个基因序列,并测序,构建进化树,分析该菌与Klebsiella variicola以及肺炎克雷伯菌序列的差异性.结果 该菌六个基因的进化树中待测菌株分别与Klebsiella variicola各参比株形成一簇,与肺炎克雷伯菌稳定区分.结论 在感染性休克患者血流中分离到1株Klebsiella variicola,其基因进化与健康人所携带的肺炎克雷伯菌显著不同.

Abstract：

Objective To identify a suspected Klebsiella variicola strain isolated from a patient with severe septic shock. Methods Sequences of rpoB, gyrA, mdh, infB, phoE and nifH genes were obtained from the clinical isolated suspected Klebsiella variicola strain, and used for constructing phylogenectic trees. Characteristics of phylogenetic trees were used to distinguish Klebsiella variicola from Klebsiella pneumoniae. Results All target gene sequences from this clinical isolated strain constantly formed a cluster with their counterparts from other Klebsiella variicola strains. These clusters were readily differentiated from Klebsiella pneumoniae clusters. Conclusions The clinical isolated strain belongs to Klebsiella variicola class and can cause septic shock. From genetic perspective, Klebsiella variicola is distinctly different from Klebsiella pneumoniae, most of which can be isolated from normal healthy people.

Identification of a suspected Klebsiella variicola strain isolated from a patient with severe septic shock

Objective To identify a suspected Klebsiella variicola strain isolated from a patient with severe septic shock. Methods Sequences of rpoB, gyrA, mdh, infB, phoE and nifH genes were obtained from the clinical isolated suspected Klebsiella variicola strain, and used for constructing phylogenectic trees. Characteristics of phylogenetic trees were used to distinguish Klebsiella variicola from Klebsiella pneumoniae. Results All target gene sequences from this clinical isolated strain constantly formed a cluster with their counterparts from other Klebsiella variicola strains. These clusters were readily differentiated from Klebsiella pneumoniae clusters. Conclusions The clinical isolated strain belongs to Klebsiella variicola class and can cause septic shock. From genetic perspective, Klebsiella variicola is distinctly different from Klebsiella pneumoniae, most of which can be isolated from normal healthy people.