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| 表没食子儿茶素没食子酸酯诱导人胃癌BGC-823细胞凋亡的机制 | |
| 引用本文： | 邹少娜,林敏,伍石华,王化修,陈波,罗招阳.表没食子儿茶素没食子酸酯诱导人胃癌BGC-823细胞凋亡的机制[J].肿瘤,2011,31(6):508-512. |
| 作者姓名： | 邹少娜 林敏 伍石华 王化修 陈波 罗招阳 |
| 作者单位： | 1. 邵阳医学高等专科学校病理教研室,邵阳,422000 2. 邵阳医学高等专科学校附属医院内科,邵阳,422000 3. 南华大学肿瘤研究所,衡阳,421001 |
| 基金项目： | 邵阳医学高等专科学校2010年度科研课题 |
| 摘 要： | 目的:探讨表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate,EGCG)对人胃癌BGC-823细胞增殖的影响及其可能的机制。方法:不同浓度EGCG单独或联合c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)抑制剂SP600125作用BGC-823细胞后,应用MTT法检测BGC-823细胞的增殖抑制率,显微镜下观察细胞的形态学改变,FCM检测细胞凋亡率,蛋白质印迹法检测细胞中p-JNK和p-c-jun蛋白的表达情况,免疫细胞化学法检测细胞中caspase-3的表达。结果:EGCG可抑制BGC-823细胞的增殖(P<0.05),呈时间和剂量依赖效应;EGCG可诱导BGC-823细胞凋亡,细胞凋亡率呈剂量依赖效应(P<0.05);EGCG可上调BGC-823细胞中p-JNK、p-c-jun和caspase-3的表达(P<0.05)。EGCG引起的BGC-823细胞增殖抑制和caspase-3表达水平的上调可被SP600125部分抑制。结论:EGCG可诱导人胃癌BGC-823细胞凋亡,其作用机制可能与激活JNK信号转导途径并上调caspase-3的表达有关。 |
| 关 键 词： | 胃肿瘤 细胞凋亡 细胞增殖 表没食子儿茶素没食子酸酯 细胞 BGC-823 |
Underlying mechanism of apoptosis of human gastric cancer cell line BGC-823 induced by epigallocatechin-3-gallate in vitro |
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| ZOU Shao-na,LIN Min,WU Shi-hua,WANG Hua-xiu,CHEN Bo,LUO Zhao-yang.Underlying mechanism of apoptosis of human gastric cancer cell line BGC-823 induced by epigallocatechin-3-gallate in vitro[J].Tumor,2011,31(6):508-512. | |
| Authors: | ZOU Shao-na LIN Min WU Shi-hua WANG Hua-xiu CHEN Bo LUO Zhao-yang |
| Institution: | ZOU Shao-na1,LIN Min2,WU Shi-hua1,WANG Hua-xiu1,CHEN Bo1,LUO Zhao-yang3 1.Department of Pathology,Shaoyang Medical College,Shaoyang 422000,China,2.Department of Medicine,Affiliated Hospital of Shaoyang Medical College,3.Institute of Cancer Research,Nanhua University,Hengyang 421001 |
| Abstract: | Objective:To investigate the effect of epigallocatechin-3-gallate(EGCG) on the proliferation of human gastric cancer BGC-823 cells and to elucidate the possible mechanism.Methods:The proliferation inhibitory rates of BGC-823 cells treated with different concentrations of EGCG alone or in combination with c-Jun N-terminal kinase(JNK) inhibitor SP600125 were detected by MTT assay.The morphological changes of BGC-823 cells were observed under a microscope.The apoptosis rate of BGC-823 cells was determined by f... |
| Keywords: | Stomach neoplasms Apoptosis Cell proliferation Epigallocatechin-3-gallate Cell BGC-823 |
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