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| Pim-3表达下调对舌鳞状细胞癌细胞凋亡的影响及其分子机制研究 | |
| 引用本文： | 焦静,刘红涛,李沙.Pim-3表达下调对舌鳞状细胞癌细胞凋亡的影响及其分子机制研究[J].肿瘤,2011,31(6):489-494. |
| 作者姓名： | 焦静 刘红涛 李沙 |
| 作者单位： | 1. 郑州人民医院口腔科,郑州,450003 2. 郑州大学生物工程系,郑州,450001 3. 郑州大学第一附属医院口腔科,郑州,450052 |
| 摘 要： | 目的:研究Pim-3表达下调对舌鳞状细胞癌细胞凋亡的影响,并探讨其凋亡的相关分子机制。方法:将Pim-3siRNA和对照siRNA分别转染舌鳞状细胞癌Tca8113细胞,另设未处理组。利用实时荧光定量PCR和蛋白质印迹法分析转染前后3组细胞中Pim-3mRNA和蛋白的表达,利用CCK-8试剂分析转染前后细胞增殖能力的变化,并进一步采用FCM法检测Pim-3表达下调对舌鳞状细胞癌Tca8113细胞凋亡的影响。最后,利用蛋白质印迹法分析转染前后细胞凋亡相关蛋白Bcl-2、Bax、Bad、pBad112、pBad136和pBad155表达的变化。结果:Pim-3siRNA能有效下调舌鳞状细胞癌Tca8113细胞中Pim-3mRNA和蛋白的表达。Pim-3表达的下调能明显抑制Tca8113细胞的增殖并诱导其凋亡。此外,Pim-3表达下调能显著上调Bax蛋白的表达和下调Bcl-2和pBad112的表达(P<0.05),但不改变总的Bad蛋白和其他Bad磷酸化形式(pBad136和pBad155)蛋白的表达。结论:Pim-3表达下调诱导的细胞凋亡可能与Bax表达水平上升及Bcl-2和pBad112表达水平下降密切相关,因而Pim-3有望成为舌鳞状细胞癌治疗的分子靶点。 |
| 关 键 词： | 舌肿瘤 基因表达调控 肿瘤 RNA干扰 原癌基因蛋白质pim-3 细胞凋亡 凋亡调控蛋白类 |
Effect of down-regulation of Pim-3 expression on apoptosis of tongue squamous-cell carcinoma and its molecular mechanism |
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| JIAO Jing,LIU Hong-tao,LI Sha.Effect of down-regulation of Pim-3 expression on apoptosis of tongue squamous-cell carcinoma and its molecular mechanism[J].Tumor,2011,31(6):489-494. | |
| Authors: | JIAO Jing LIU Hong-tao LI Sha |
| Institution: | JIAO Jing1,LIU Hong-tao2,LI Sha3 1.Department of Stomatology,Zhengzhou People's Hospital,Zhengzhou 450003,China,2.Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,3.Department of Stomatology,First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052 |
| Abstract: | Objective:To investigate the effect of down-regulation of Pim-3 expression mediated by RNA interference on apoptosis of tongue squamous-cell carcinoma,and to explore its molecular mechanism.Methods:Pim-3 siRNA and single siRNA were transfected into tongue squamous-cell carcinoma Tca8113 cells,respectively.The expressions of Pim-3 mRNA and protein were detected by real-time fluorescence quantitative PCR and Western blotting.The change of cell proliferation capability was analyzed using CCK-8 kit.The effect o... |
| Keywords: | Tongue neoplasms Gene expression regulation neoplastic RNA interference Proto-oncogene proteins pim-3 Apoptosis Apoptosis regulatory proteins |
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