COX-2和VEGF在前列腺癌中的表达及其与肿瘤Gleason评分之间关系的研究

目的探讨环氧化酶-2(COX-2)和血管内皮生长因子(VEGF)在前列腺癌(PCa)中的表达及其与肿瘤Gleason评分之间的关系。方法30例前列腺癌组织,按Gleason评分,将≥7分者列为高Gleason评分组(n=10);<7分者列为低Glea-son评分组(n=20)。良性前列腺增生(BPH)20例,BPH并高级前列腺上皮内瘤(PIN)12例。正常前列腺组织(NP)10例。应用免疫组化方法检测各组织中COX-2、VEGF的表达水平及其微血管密度(MVD)。结果PCa、PIN中COX-2、VEGF的阳性表达率及MVD值高于BPH及NP(P均<0.05),高Gleason评分组中COX-2、VEGF的阳性表达率(81.3%、93.8%)及MVD值(63.4±1.80)高于低Gleason评分组(42.9%、64.3%、51.7±1.56,P<0.05)。结论COX-2、VEGF在前列腺癌中均为高表达,且与肿瘤的Gleason评分密切相关。

Expression of COX-2 and VEGF in human prostate cancer and their relationship with Gleason score

Objective Investigating the expression of Cycloxygenase-2(COX-2)and vascular endothelial growth factor(VEGF) in human prostate cancer(PCa) to explore their relationship with tumor angiogenesis in PCa.Methods Specimens from 72 patients with PCa(30 cases),benign prostatic hyperplasia(BPH,20 cases),high-grade prostate intraepithelial neoplasia(PIN,12) and normal prostate(NP,10 cases) were examined.The PCa was divided into high Gleason score group(Gleason score≥7) and low Gleason score group(Gleason score<7).The expression of COX-2,VEGF and the microvessel density(MVD) were detected by means of immunohistochemistry.Results The positive rates of COX-2,VEGF and MVD in PCa and PIN were higher than those in BPH and NP(all P<0.05),and the positive rates of COX-2 and VEGF and MVD were higher in high Gleason score group than those in low Gleason score group(all P<0.05).Conclusion The high expressions of COX-2,VEGF in PCa may be related to tumor pathological grade.