| HT036是纤维化相关基因P311的相互作用蛋白 |
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| 引用本文： | 彭旭,谭江琳,袁顺宗,贺伟峰,陈烯伟,罗高兴,吴军.HT036是纤维化相关基因P311的相互作用蛋白[J].创伤外科杂志,2009,11(5):412-415. |
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| 作者姓名： | 彭旭 谭江琳 袁顺宗 贺伟峰 陈烯伟 罗高兴 吴军 |
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| 作者单位： | 第三军医大学西南医院全军烧伤研究所,创伤、烧伤与复合伤国家重点实验室、疾病蛋白质组学重庆市市级重点实验室,重庆,400038 |
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| 基金项目： | 国家自然科学基金面上项目(30571922;30672174) 创伤烧伤复合伤国家重点实验室自主研究课题项目资助 |
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| 摘 要： | 目的利用激光共聚焦显微镜观察未知蛋白（HT036）与纤维化相关蛋白（P311）的细胞内表达与共定位。方法构建带绿色荧光的HT036真核表达载体，经鉴定正确后，与前期构建带红色荧光的P311真核表达载体共转染人胚肾上皮细胞株（HEK293），同时共转染空载体做对照，激光共聚焦显微镜下观察蛋白在细胞内表达、分布和共定位。结果HT036真核表达载体经聚合酶链反应（PCR）、双酶切和测序鉴定正确，与P311共转染HEK293细胞后，激光共聚焦显微镜观察到绿色和红色荧光表达。HT036和P311主要分布在细胞胞浆，两者在HEK293细胞中存在共定位，而对照组则无该现象。结论通过酵母双杂交筛选出的P311相互作用蛋白HT036，在活体细胞内存在空间上的共定位，进一步证明它们之间的相互作用，为探讨P311纤维化机制提供新的切人点。
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| 关 键 词： | 创伤修复 蛋白 基因 成纤维细胞 瘢痕 |
HT036 as the novel interactive protein of fibrotic-related P311 |
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| PENG Xu,TAN Jiang-lin,YUAN Shun-zong,et al..HT036 as the novel interactive protein of fibrotic-related P311[J].Journal of Traumatic Surgery,2009,11(5):412-415. |
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| Authors: | PENG Xu TAN Jiang-lin YUAN Shun-zong |
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| Institution: | PENG Xu,TAN Jiang-lin,YUAN Shun-zong,et al.(Chongqing Key Laboratory of Proteomics of Diseases,State Key Laboratory of Trauma,Burns , Combined Injury,Institute of Burns,Southwest Hospital,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To observe the intracellular expression and the co-location of HT036 with P311 in human embryo kidney cell line.Methods The encoding sequence of HT036 was amplified and cloned into the eukaryotic expression plasmid pEGFP-N2.The construction was verified by sequencing and then co-transfected into HEK293 with pDsRed-P311.The expression products were visualized and analyzed by confocal microscope.Results The vector was successfully constructed.The confocal microscope scanning showed that the fluoresc... |
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| Keywords: | trauma repair protein gene fibroblast scar |
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