| 人肝癌组织中原癌基因Pokemon的表达及其临床意义 |
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| 引用本文： | 王波,田德安,张全乐,夏丽敏,晏维,王颖,刘梅. 人肝癌组织中原癌基因Pokemon的表达及其临床意义[J]. 胃肠病学和肝病学杂志, 2010, 19(2) |
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| 作者姓名： | 王波 田德安 张全乐 夏丽敏 晏维 王颖 刘梅 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院消化内科,湖北,武汉,430030 |
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| 摘 要： | 目的 探讨肝癌组织中Pokemon基因的表达,并分析其与肿瘤的大小、分化程度、TNM分期等临床资料的相关性.方法 收集30例原发性肝癌患者的肿瘤及其癌旁组织,用逆转录酶聚合链反应(RT-PCR)及免疫印迹(Western blot)的方法分别从核酸及蛋白水平检测标本中Pokemon基因的表达,并对其与患者的性别、肿瘤大小、淋巴结转移、肿瘤分化程度、肿瘤的TNM分期等临床资料进行统计分析.结果 RT-PCR检测肝癌和癌旁组织mRNA表达的相对强度分别为0.71±0.03、0.17±0.01.Western blot检测蛋白水平的相对表达强度分别为10.64±0.92、1.06±0.18,两者比较有统计学意义(P<0.05),肝癌组织中Pokemon的表达与肿瘤的大小有关,而与患者的年龄、肿瘤的分化程度、淋巴结转移及肿瘤TNM分期等无关.结论 Pokemon的表达上调在肝癌的增殖中可能发挥重要作用.
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| 关 键 词： | 肝癌 原癌基因 |
Expression of Pokemon and its clinical significance in human hepatocellular carcinoma |
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| WANG Bo,TIAN De'an,ZHANG Quanle,XIA Limin,YAN Wei,WANG Ying,LIU Mei. Expression of Pokemon and its clinical significance in human hepatocellular carcinoma[J]. Chinese Journal of Gastroenterology and Hepatology, 2010, 19(2) |
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| Authors: | WANG Bo TIAN De'an ZHANG Quanle XIA Limin YAN Wei WANG Ying LIU Mei |
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| Affiliation: | WANG Bo,TIAN De'an,ZHANG Quanle,XIA Limin,YAN Wei,WANG Ying,LIU Mei Department of Gastroenterology,Affiliated Tongji Hospital of Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030,China |
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| Abstract: | Objective To explore the expression of Pokemon in human hepatocellular carcinoma and its correlation with clinical features.Methods RT-PCR and Western blot were applied to detect the expression of Pokemon in 30 cases of resection samples and their corresponding paracancerous samples.Correlation between expression of Pokemon and clinical features was analyzed.Results The expressions of Pokemon mRNA and protein were significantly higher in the cancerous tissues than those in the paracancerous tissues (P<0.05)... |
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| Keywords: | Pokemon |
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