气相色谱法测定复方乳酸钠山梨醇注射液中的山梨醇含量

目的：建立山梨醇的毛细管气相色谱分析方法。方法：山梨醇经乙酸酐衍生化为沸点较低的山梨醇六乙酯,用HP-5毛细管色谱柱分离,火焰离子化检测器（FID）进行检测。色谱柱温度为220℃、进样口和检测器的温度均为290℃,氮气流速3 ml/min。结果：优化了样品衍生化和色谱分析条件,实现了杂质与山梨醇的分离,排除了杂质对山梨醇测定的干扰。本方法的线性范围为0~60 mg/ml,检出限为0.01 mg/ml,日内精密度RSD为0.081%,日间精密度RSD为0.749%~0.872%,平均回收率为99.8%~100.0%,RSD为0.049%。结论：本方法操作简单、灵敏、可靠,能满足山梨醇定量分析的要求。

Determination of D -sorbitol in compound sodium lactate and sorbitol injection by capillary gas chromatography

Objective： To develop a capillary gas chromatographic method for the determination of D-sorbitol.Methods： The derivative of sorbitol,sorbital hexacetatate,the boiling point of which is lower than that of its origin,was separated by an HP-5 capillary chromatographic column at 220 ℃ and determined with an ionization detector（FID）.The temperature of the detector and the inlet of samples was 290 ℃,and the nitrogen flow rate was of 3 ml/min.Results： The conditions for both the derivatization of sorbitol and the chromatographic separation were optimized and sorbitol was completely separated from impurities.The linear range was 0~60 mg/ml and the limit of detection of was 0.01 mg/ml.The precisions of within-day and between-days were 0.081% and 0.749% ~0.872% respectively.The average recovery was 99.8%~100.0% and average RSD was 0.049%.Conclusions： The method developed in the present study is simple,sensitive and reliable for quantitative analysis of sorbitol.