| 单抗ELISA竞争法在测定激素中的应用研究 |
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| 引用本文： | 邢冬红,潘菊芬,黄焕军,缪慧.单抗ELISA竞争法在测定激素中的应用研究[J].天津医科大学学报,2001,7(2):199-200,203. |
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| 作者姓名： | 邢冬红 潘菊芬 黄焕军 缪慧 |
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| 作者单位： | 1. 天津医科大学医学检验系免疫教研室,
2. 天津医科大学基础医学院 |
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| 基金项目： | 天津市教委重点发展学科资助课题(编号:98Q08) |
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| 摘 要： | 目的：用人绒毛膜促性腺激素（hCG)，进行ELISA竞争法测定激素的方法研究。方法：用ELISA竞争法检测尿中hCG。以抗hCG单克隆抗体包被酶标板孔，先后加入含有待测hCG的尿标本及酶标记的hCG，使二者共同竞争与板孔的单抗结合，经显色后按颜色深浅、DB值大小判断阴、阳性。结果：经对临床近100份标本检测结果证明本法与乳胶法判断的结果符合率为97％。结论：ELISA竞争法也可用于检测激素及其它小分子抗原，方法简便、省时。
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| 关 键 词： | ELISA竞争法 抗hCG 妊娠诊断 |
| 文章编号： | 1006-8147(2001)02-0199-03 |
The observation of competitive ELISA for detection of hormone |
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| XING Dong-hong ,PAN Ju-fen ,HUANG Huan-jun ,MIAO Hui.The observation of competitive ELISA for detection of hormone[J].Journal of Tianjin Medical University,2001,7(2):199-200,203. |
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| Authors: | XING Dong-hong PAN Ju-fen HUANG Huan-jun MIAO Hui |
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| Institution: | XING Dong-hong 1,PAN Ju-fen 2,HUANG Huan-jun 2,MIAO Hui 2 |
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| Abstract: | Objective: To observe the method for detecting hormones we used hCG with competitive ELISA. Methods: Described here is a competitive ELISA for detecting hCG in urine.Anti-hCG monoclonal antibodies recognizing different epitopes were coated in microplate wells; the urine sample and hCG-peroxidase conjugate were added to the wells in order. After OPD was added,the deep or light colors (or different OD value) showed the negative or positive resaffs respectively.Results:The results of about 100 clinical urine samples showed that the result of the method correlated well to the traditional latex agglutination inhibition test,the correlation rate is 97%.Conclusion: Competitive ELISA can also be used to determine hormones and other small molecular antigens,and it is easy and quick to perform. |
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| Keywords: | Toxoplasma gondii Cyst Chronic infection |
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