牙周致病菌在冠心病患者龈下菌斑中的分布

目的:分析牙周致病菌在冠心病患者龈下菌斑中的分布情况,及冠心病患者龈下菌斑中牙周致病菌的分布与慢性牙周炎(chronic periodontitis,CP)病变程度之间的关系。方法:收集44例患冠心病并伴有CP患者的龈下菌斑,采用Chelex-100法提取细菌DNA,以聚合酶链反应(polymerase chain reaction,PCR)法检测龈下菌斑中牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)、伴放线菌嗜血菌(Haemophilus actinomycetemcomitans,Ha)、具核梭杆菌(Fusobacterium nucleatum,Fn)、中间普氏菌(Prevotella intermedia,Pi)、福赛斯坦纳菌(Tannerella forsythensis,Tf)五种牙周炎相关致病菌。结果:44例冠心病患者的龈下菌斑中各牙周致病菌的检出率分别为:Pg 19(43.18%)、Ha 9(20.45%)、Pi 27(61.36%)、Fn 38(86.36%)、Tf 41(93.18%);轻度CP患者1例,Fn 1例(100%);中度CP患9例,其中Pg6(66.67%)、Ha 2(22.22%)、Pi 7(77.78%)、Fn 9(100%)、Tf 8(88.89%);重度CP患者34例,其中Pg13(38.24%)、Ha 7(20.59%)、Pi 20(58.82%)、Fn 28(82.35%)、Tf 33(97%)。结论:Tf、Fn可能在冠心病的发生发展中起着重要作用,冠心病患者中CP的发病和进展可能有其特定的细菌学病因。

Distribution of Periodontal Pathogens in the Subgingival Plaque of Patients with Coronary Heart Disease

Objective: To investigate the prevalence of periodontal pathogens in the subgingival plaque of patients with coronary heart disease,and the relationship between the distribution of periodontal pathogens and various degrees of chronic periodontitis(CP).Methods: Subgingival plaque samples were harvested from a total of 44 CP patients with coronary heart disease.The bacteria DNA was obtained from subgingival plaque samples using the chelex-100 method.The extracted DNA Porphyromonas gingivalis (Pg),Haemophilus actinomycetemcomitans(Ha),Fusobacterium nucleatum(Fn),Prevotella intermedia(Pi),Tannerella forsythensis,(Tf)] was examined using the polymerase chain reaction(PCR) technique by means of specific oligonucleotide primers for periodontal bacteria.Results: In the subgingival plaques samples of 44 patients,19 Pg(43.18%),9 Ha(20.45%),27 Pi(61.36%),38 Fn(86.36%) and 41 Tf(93.18%) were detected.One patient was initial CP,only Fn(100%) were detected.Nine patients were moderate CP,6 Pg(66.67%),2 Ha((22.22%)), 7 Pi(77.78%),9 Fn(100%) and 8 Tf(88.89%) were detected.Thirty-eight patients were severe CP,13 Pg((38.24%)),7 Ha(20.59%),20 Pi(58.82%),28 Fn(82.35%) and 33 Tf(97%) were detected.Conclusion: The result indicated that Tf and Fn may play important roles in the pathogenesis of coronary heart disease and certain subgingival bacteria harvested from patients with coronary heart disease may play important roles in the pathogenesis of periodontal disease.