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沙棘总黄酮的大鼠肠吸收特性考察
引用本文:申雪丽,袁勇,黄川生,王新春,尹俊涛. 沙棘总黄酮的大鼠肠吸收特性考察[J]. 中国实验方剂学杂志, 2013, 19(18): 61-64
作者姓名:申雪丽  袁勇  黄川生  王新春  尹俊涛
作者单位:1. 石河子大学药学院,新疆石河子,832002
2. 石河子大学医学院一附院,新疆石河子,832008
3. 石河子大学药学院,新疆石河子832002;石河子大学医学院一附院,新疆石河子832008
4. 石河子开发区神内食品有限公司,新疆石河子,832000
基金项目:新疆建设兵团火炬计划项目(2011BD021);国家自然科学基金-新疆联合基金重点项目(U1203204)
摘    要:目的:考察沙棘总黄酮在大鼠小肠的吸收特性.方法:运用大鼠在体单向灌流和离体外翻肠囊模型,采用HPLC测定槲皮素、山奈酚、异鼠李素含量,计算沙棘总黄酮在大鼠小肠的吸收参数.结果:沙棘总黄酮中槲皮素、山奈酚、异鼠李素的最佳吸收部位分别为回肠、回肠、十二指肠,吸收速率常数(Ka)分别为3.405×10-2,3.649×10-2,5.671×10-2,沙棘总黄酮质量浓度在50 ~200 mg·L-1时,Ka和表观吸收系数无显著性差异.累积吸收量随药物质量浓度升高呈线性增加.结论:沙棘提取物中3种黄酮类成分均呈现一级动力学特征,吸收机制均为被动扩散,且在小肠有特定吸收部位.

关 键 词:沙棘总黄酮  槲皮素  山奈酚  异鼠李素  吸收机制
收稿时间:2013-02-18

Investigation of Rat Intestinal Absorption Properties of Total Flavonoids from Hippophae Fructus
SHEN Xue-li,YUAN Yong,HUANG Chuan-sheng,WANG Xin-chun and YIN Jun-tao. Investigation of Rat Intestinal Absorption Properties of Total Flavonoids from Hippophae Fructus[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(18): 61-64
Authors:SHEN Xue-li  YUAN Yong  HUANG Chuan-sheng  WANG Xin-chun  YIN Jun-tao
Affiliation:College of Pharmacy, Shihezi University, Shihezi 832002, China;First Affiliated Hospital of Medical College, Shihezi University, Shihezi 832008, China;First Affiliated Hospital of Medical College, Shihezi University, Shihezi 832008, China;College of Pharmacy, Shihezi University, Shihezi 832002, China;First Affiliated Hospital of Medical College, Shihezi University, Shihezi 832008, China;Shihezi Development Zone Shennei Food Co. Ltd, Shihezi 832000, China
Abstract:Objective: To investigate rat intestinal absorption characteristics of total flavonoids from Hippophae Fructus. Method: In vitro everted gut sac model and in situ rat single-pass perfusion model were adopted,the contents of quercetin,kaempferol and isorhamnetin were determined by HPLC,rat intestinal absorption parameters of total flavonoids from Hippophae Fructus were calculated. Result: The best absorption parts of quercetin,kaempferol,isorhamnetin was ileum,ileum and duodenum,respectively.Absorption rate constant (Ka) of these three ingredients were 3.405×10-2,3.649×10-2,5.671×10-2,respectively.Ka and apparent absorption coefficient had no significant difference when the concentration of total flavonoids from Hippophae Fructus was 50-200 mg·L-1.A positive correlation was found between drug concentration and accumulated absorption amount. Conclusion: Absorption characteristics of these three main flavonoids components were consistent,all complied with the first order absorption kinetics,absorption mechanisms of them were passive diffusion,and they had specific absorption site in small intestine.
Keywords:total flavonoids from Hippophae Fructus  quercetin  kaempferol  isorhamnetin  absorption mechanism
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