多孔壳聚糖膜的专一性酶法制备及性能表征

目的:制备多孔医用壳聚糖膜并进行性能表征.方法:采用溶剂蒸发凝胶法制膜,通过内切壳聚糖酶对膜原位降解制备多孔壳聚糖膜,利用扫描电镜观察其形态,称重法检测其吸湿保湿性与酶解时间-余重关系,检测多孔壳聚糖膜对牛血清白蛋白的吸附性和对磺胺嘧啶银的控缓释性能.结果:壳聚糖与内切酶的比例0.1 g·U-1,酶解时间3h,膜表面及内部均产生了适用于药物载体的多孔结构.结论:通过内切酶酶解原位成孔的壳聚糖膜具有反应条件温和、致孔率可控、无毒性物质残留等优点,具有良好的应用前景.

Specificity Enzymatic Preparation and Performance Characterization of Porous Chitosan Membranes

Objective: To prepare porous medical chitosan membranes and do their performance characterization. Method: Membranes were prepared by solvent evaporation gel method,degradation of chitosan enzyme to prepare porous chitosan membranes,then their morphology was observed by scanning electron microscope,weighing method was adopted to detect relationship between moisture absorption effect and hydrolysis time-remaining weight,and test adsorption of porous chitosan membranes to bovine serum albumin and controlled release properties to silver sulfadiazine. Result: Ratio of chitosan and endonuclease was 0.1 g·U^-1,enzymolysis time was 3 h,both surface and internal of membranes generated porous structure which was suitable for pharmaceutical carriers. Conclusion: In situ endonuclease digestion of porous chitosan membranes had advantages of mild reaction conditions,pore rate controlled and non-toxic substance residues etc,which had a good application prospect.