| HPLC测定龙须藤中2种多甲氧基黄酮的含量 |
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| 引用本文： | 易荆丽,张嘉家,周毅生,赵永恒,王嵩.HPLC测定龙须藤中2种多甲氧基黄酮的含量[J].中国实验方剂学杂志,2013,19(18):155-157. |
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| 作者姓名： | 易荆丽 张嘉家 周毅生 赵永恒 王嵩 |
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| 作者单位： | 广东药学院,广州,510006 |
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| 摘 要： | 目的:建立HPLC测定龙须藤中5,7,3’,4’,5’-五甲氧基黄酮和5,6,7,5’-四甲氧基-3’,4’-亚甲二氧基含量的方法.方法:DIKMA PlatisilODS C18色谱柱(4.6 mm ×250 mm,5μm),流动相乙腈(A)-0.1％磷酸溶液(B),梯度洗脱(0 min,25％ A;15 min,45％A；40 min,50％ A),流速0.8 mL· min-,检测波长324 nm.结果:5,7,3’,4’,5’-五甲氧基黄酮和5,6,7,5’-四甲氧基-3',4'-亚甲二氧基分别在0.0084～0.3360 μg(r =0.999 9),0.013 4～0.536 0 μg(r =0.999 7)与峰面积呈良好的线性关系,平均回收率分别为99.41％,98.65％,RSD分别为0.57％,1.01％.结论:该方法操作简单准确,分离效果、精密度和重复性好,适合于测定该药材中5,7,3’,4’,5’-五甲氧基黄酮和5,6,7,5’-四甲氧基-3’,4’-亚甲二氧基的含量.
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| 关 键 词： | 高效液相色谱 龙须藤 多甲氧基黄酮 含量测定 |
| 收稿时间： | 2012/10/24 0:00:00 |
Determination of Two Polymethoxylated Flavonoids in Bauhinia championii by HPLC |
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| YI Jing-li,ZHANG Jia-ji,ZHOU Yi-sheng,ZHAO Yong-heng and WANG Song.Determination of Two Polymethoxylated Flavonoids in Bauhinia championii by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2013,19(18):155-157. |
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| Authors: | YI Jing-li ZHANG Jia-ji ZHOU Yi-sheng ZHAO Yong-heng and WANG Song |
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| Institution: | Guangdong Pharmaceutical University, Guangzhou 510006, China;Guangdong Pharmaceutical University, Guangzhou 510006, China;Guangdong Pharmaceutical University, Guangzhou 510006, China;Guangdong Pharmaceutical University, Guangzhou 510006, China;Guangdong Pharmaceutical University, Guangzhou 510006, China |
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| Abstract: | |
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| Keywords: | HPLC Bauhinia championii polymethoxylated flavonoids quantitative determination |
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