合欢花总黄酮对抑郁模型大鼠海马CA3区BDNF和TrkB表达的影响

目的:观察合欢花总黄酮对抑郁模型大鼠海马CA3区脑源性神经营养因子(BDNF)及其受体酪氨酸激酶B(TrkB)表达的影响.方法:将90只SD大鼠随机分为正常组、模型组、盐酸文拉法辛组(12.5 mg·kg-1)、合欢花总黄酮(100,50,25 mg·kg-1)剂量组.应用孤养加慢性不可预见性应激建立抑郁症模型,造模同时ig给药,每天1次,连续给药21 d,正常组、模型组ig等体积蒸馏水.用Morris水迷宫法测定各组大鼠学习记忆能力,免疫组织化法检测大鼠海马CA3区BDNF及TrkB表达.结果:与正常组比较,模型组大鼠Morris水迷宫试验逃避潜伏期时间增加、成功次数减少(P ＜0.05,P＜0.01)；海马CA3区BDNF及受体TrkB的表达降低(P＜0.01).与模型组比较,盐酸文拉法辛组、合欢花总黄酮3个剂量组Morris水迷宫试验逃避潜伏期时间缩短、成功次数增加(P＜ 0.05或P＜0.01),海马CA3区BDNF及受体TrkB的表达明显增高(P＜0.05或P＜0.01).结论:合欢花总黄酮能够提高抑郁模型大鼠学习记忆能力,其作用机制可能与增加抑郁模型大鼠海马CA3区BDNF及其受体TrkB的表达,进而保护海马神经元有关.

Study on Antidepressant Effect of Albizzia julibrissin Flower Total Flavonoids on the Expression of BDNF and TrkB in the Hippocampus CA3 of Rats with Depression

Objective: To research the effects of Albizzia julibrissin flower total flavonoids (AJFTF) on the expression of brain-derived neurotrophic factor(BDNF) and tyrosin kinase B(TrkB) in the hippocampus CA3 of rats with depression. Method: Ninty SD rats were randomly divided into normal group, model group, venlafaxine hydrochloride group (12.5 mg·kg^-1), and high, middle, low dosages of AJFTF groups(100,50,25 mg·kg^-1). Depression model rats were induced by solitary cultivation plus chronic stress. Rats in venlafaxine hydrochloride and AJFTF groups were orally administered with venlafaxine hydrochloride or AJFTF once daily for 21 days, and rats in normal and model groups with equal volume distilled water. The learning and memory ability of rats were determined by Morris maze method, and the expressions of BDNF and Trkb in hippocampal CA3 were determinated by immunohisto chemical assay. Result: Compared with the normal group, escape latencies in model group were significantly increased and the times of succeed were decreased (P<0.05 or P<0.01), and the expression of BDNF and TrkB in the hippocampus CA3 were significantly decreased (P<0.01). Compared with the model group, escape latencies were significantly decreased and the times of succeed were increased (P<0.05 or P<0.01)in venlafaxine hydrochloride group, AJFTF (100,50,25 mg·kg^-1) dosage groups, and the expression of BDNF and TrkB in the hippocampus CA3 was significantly increased (P<0.05 or P<0.01). Conclusion: AJFTF can improve the learning and memory ability of rats with depression, and its mechanism may be related to the improvement of the expression of BDNF and TrkB in the hippocampus CA3 to protect the hippocampal neurons.