首页 | 本学科首页   官方微博 | 高级检索  
     

雏鸡基底乳头庆大霉素损伤后TGFβ1、TGFβ2、TGFβ3的表达
引用本文:李华伟,汪吉宝. 雏鸡基底乳头庆大霉素损伤后TGFβ1、TGFβ2、TGFβ3的表达[J]. 临床耳鼻咽喉头颈外科杂志, 1998, 12(10): 463-465
作者姓名:李华伟  汪吉宝
作者单位:同济医科大学附属协和医院耳鼻咽喉科!武汉,430022,上海医科大学眼耳鼻咽喉医院耳鼻咽喉科,200031
摘    要:应用免疫组化技术,检测雏鸡基底乳头庆大霉素损伤后1、3、7、14、21和28d时TGFβ1、TGFβ2、TGFβ3的表达情况。结果显示,在雏鸡基底乳头庆大霉素损伤后修复过程中,可见修复的早期TGFβ2、TGFβ3有明显表达。

关 键 词:毛细胞损伤 再生 β转化生长因子 庆大霉素

Study on TGF beta 1, TGF beta 2, TGF beta 3 expression in the chick basilar papilla following gentamicin toxicity]
Li Huawei. Study on TGF beta 1, TGF beta 2, TGF beta 3 expression in the chick basilar papilla following gentamicin toxicity][J]. Journal of clinical otorhinolaryngology, head, and neck surgery, 1998, 12(10): 463-465
Authors:Li Huawei
Affiliation:Union Hospital of Tognji Medical University, Wuhan 430022.
Abstract:The beta-type transforming growth factors (TGF beta s) are secreted proteins, which play an important role in regulation of cell proliferation and differentiation in the embryonic inner ear. In order to probe into the effect of TGF beta s on the hair cell regeneration, expression of TGF beta 1, TGF beta 2 and TGF beta 3 proteins were examined by using immunohistochemistry in the chicken basilar papilla during hair cell regeneration following gentamicin ototoxicity. Ten-day-old chickens received daily subcutaneous injection of gentamicin sulfate 50 mg/kg of ten consecutive days. The animals were allowed to survive 1,3,7,14,21 and 28 days before sacrifice and preparation for examination of the expression of TGF beta 1, TGF beta 2 and TGF beta 3 proteins. Immunostaining results demonstrated that TGF beta 2 and TGF beta 3 proteins were observed in the damaged region of basilar papilla. TGF beta 2 and TGF beta 3 proteins positive cells were limited to the lumenal nuclear layer within the damaged region. TGF beta 1 protein positive cell was not found in our study. These results indicated that TGF beta 2 and TGF beta 3 proteins might play a role in regulating proliferation of the supporting cells immigrated into the lumenal nuclear layer during hair cell regeneration.
Keywords:
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号