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| 心肌M2胆碱受体抗体对大鼠心房及心室肌cAMP含量的影响 | |
| 引用本文： | 朱琳,李香珍,冯羡菊,王文泽,张琳,陈爱莲.心肌M2胆碱受体抗体对大鼠心房及心室肌cAMP含量的影响[J].郑州大学学报(医学版),2001,36(3). |
| 作者姓名： | 朱琳 李香珍 冯羡菊 王文泽 张琳 陈爱莲 |
| 作者单位： | 1. 河南医科大学第一附属医院药剂科 2. 河南医科大学第一附属医院检验科 3. 山西医科大学第二附属医院心外科 4. 河南省人民医院检验科 5. 濮阳市中医院内科 |
| 基金项目： | 国家自然科学基金资助项目!3 93 70 3 0 4 |
| 摘 要： | 目的 :了解心肌M2 胆碱受体抗体 (M2 Ab)对大鼠心房和心室肌cAMP含量的影响 ,并与M2 胆碱受体激动剂卡巴可 (Carb)的作用进行了对比观察。方法 :采用离体生化放射免疫分析法测定M2 Ab及M2 胆碱受体激动剂Carb对心肌cAMP含量的影响。结果 :①M2 Ab及Carb两者均可剂量依赖性抑制异丙肾上腺素 (Isoproterenol,Iso)所刺激的大鼠心房及心室肌cAMP的增加。卡巴可浓度为 2 μmol/L ,10 μmol/L ,5 0 μmol/L时可分别抑制Iso所刺激的cAMP含量 (8.5± 1.2 ) % ,(16 .2± 1.4) % ,(2 9.5± 2 .1) % ,而M2 Ab浓度为 5 0nmol/L ,10 0nmol/L ,40 0nmol/L时 ,可分别抑制 (6 .1± 0 .6 ) % ,(17.3± 1.8) % ,(31.7± 3.1) % (P <0 .0 1)。②Carb(10 μmol/L)及M2 Ab(10 0nmol/L)两者可分别抑制基础cAMP(49.2± 4.3) %和 (6 4.3± 5 .1) %。③M受体阻断剂阿托品 (Atr) (1.5 μmol/L)不但可阻断Carb对Iso的抑制反应 ,亦能阻断M2 Ab的这种反应。而相应的抗原性肽段也能阻断M2 Ab的这种反应。结论 :M2 Ab抑制Iso引起的心室肌细胞cAMP生成量的增加反应 ,类似于M受体激动剂Carb ,两者效应均通过作用于M2 受体途径实现。 |
| 关 键 词： | 抗肽抗体 M2胆碱受体 cAMP |
Effects of anti-peptide antibody against human M2 muscarinic receptors on the cAMP content in rat ventricles |
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| ZHU Lin ,LI Xiangzhen ,FENG Xianju ,WANG Wenze ,ZHANG Lin ,CHEN Ailian.Effects of anti-peptide antibody against human M2 muscarinic receptors on the cAMP content in rat ventricles[J].Journal of Zhengzhou University: Med Sci,2001,36(3). | |
| Authors: | ZHU Lin LI Xiangzhen FENG Xianju WANG Wenze ZHANG Lin CHEN Ailian |
| Institution: | ZHU Lin 1),LI Xiangzhen 1),FENG Xianju 2),WANG Wenze 2),ZHANG Lin 3),CHEN Ailian 4) 1) Department of Pharmacy,the First Affiliated Hospital,Henan Medical University,Zhengzhou 450052\ 2)Department of Check Out,the First Affi |
| Abstract: | Aim:To study the effects of anti-peptide antibodies(M2-Ab) against the second extracellular loop of human muscarinic receptor 2 on the cAMP content in rat atria and ventricles.These effects were compared with those of the muscarinic receptor agonist carbachol (Carb).Methods:Radioimmunoassay technique,was used for determining the effects of anti-pepitide antibodies and carb on cAMP content in rat ventricles.Result:①both Carb and M2-Ab were able to inhibit the isoproterenol (Iso) stimulated cAMP production in rat atria and ventricles.Carb at 2μmol/L,10μmol/L and 50μmol/L decreased Iso-stimulated cAMP production by (8.5±1.2)%，(16.2±1.4)% and (29.5±2.1)%，respectively;whereas M2-Ab at 50nmol/L,100nmol/L and 400nmol/L decreased it by (6.1±0.6)%,(17.3±1.8)% and (31.7±3.1)% (P＜0.01),respectively.②Both Carb and M2-Ab could also inhibit the basal cAMP content significantly.Carb at 10μmol/L and M2-Ab at 100nmol/L decreased it by (49.2±4.3)% and (64.3±5.1)%,respectively.③The inhibitory effects of both M2-Ab and Carb on Iso-stimulated cAMP production were significantly prevented by atropine at 1.5μmol/L.The inhibitory effect of M2-Ab at 100nmol/L was almost completely abolished by the peptide (700 nmol/L) itself.Conclusion:The anti-M2 receptor antibodies display a stimulatory muscarinic activity similar to muscarinic receptor agonist,carbachol,in inhibiting isoproterenol stimulated cAMP production of ventricles. |
| Keywords: | anti peptide antibody M 2 muscarinic receptor cAMP |
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