| 苦参碱诱导大鼠肝星状细胞凋亡的体外研究 |
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| 引用本文： | 翁山耕,胡雨云,田雄英,林丽娟,林永堃.苦参碱诱导大鼠肝星状细胞凋亡的体外研究[J].福建医科大学学报,2009,43(6):443-446. |
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| 作者姓名： | 翁山耕 胡雨云 田雄英 林丽娟 林永堃 |
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| 作者单位： | 福建医科大学附属第一医院肝胆胰外科,福建省腹部外科研究所,福州350004 |
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| 摘 要： | 目的研究苦参碱对体外培养的大鼠肝星状细胞系rHSC-99凋亡的影响,并探讨其机制。方法体外培养rHSC-99,随机分成4组：对照组（A组）,苦参碱0.12 mg/kg组（B组）,苦参碱0.24 mg/kg组（C组）,苦参碱0.48 mg/kg组（D组）。苦参碱作用24 h后,TUNEL法检测rHSC-99凋亡,半定量RT-PCR方法及免疫细胞化学方法检测rHSC-99中神经生长因子（NGF）,p75及Caspase-3的表达情况。结果不同浓度的苦参碱干预rHSC-99 24 h后,rHSC-99凋亡率随苦参碱的浓度增大而增高,D组凋亡率最高（P〈0.01）;NGF,p75,Caspase-3mRNA及其蛋白表达随苦参碱的浓度加大而表达升高,C组表达最高（P〈0.01）。结论苦参碱可诱导HSCs凋亡。激活NGF/p75通路、上调Caspase-3蛋白表达可能是苦参碱诱导肝星状细胞凋亡的机制之一。
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| 关 键 词： | 苦参碱 星形细胞 肝硬化 细胞凋亡 神经生长因子类 神经组织蛋白质类 基因表达 |
A Study on the Apoptosis of Rat Hepatic Stellate Cells Induced by Matrine in vitro |
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| WENG Shangeng,HU Yuyun,TIAN Xiongying,LIN Lijuan,LIN Yongkun.A Study on the Apoptosis of Rat Hepatic Stellate Cells Induced by Matrine in vitro[J].Journal of Fujian Medical University,2009,43(6):443-446. |
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| Authors: | WENG Shangeng HU Yuyun TIAN Xiongying LIN Lijuan LIN Yongkun |
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| Institution: | WENG Shangeng,HU Yuyun,TIAN Xiongying,LIN Lijuan,LIN YongkunHepatopancreatobiliary Surgery Department,The First Affiliated Hosptial,Fujian Medical University,Institute of Abdominal Surgical Research,Fuzhou 350005,China |
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| Abstract: | Objective To investigate the effect of matrine on inducing apoptosis of rat hepatic stellate cell line-99(rHSC-99) in vitro and to decipher the underlying mechanism.Methods rHSC-99 cells were treated with matrine of different concentration of 0.12,0.24 and 0.48 mg/mL for 24 h respectively.The induced apoptosis of HSC was detected by Terminal deoxynucleotidy transferase mediated dUTP nick end labeling(TUNEL).RT-PCR and immuocytochemistry were used to detect the mRNA and protein level of NGF,p75,and Caspase-3... |
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| Keywords: | matrine astrocytes liver cirrhosis apoptosis nerve growth factors nerve tissue proteins gene expression |
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