| Effect of Exogenous bFGF on the Proliferation of Human Adenoid Cystic Carcinoma ACC-2 Cells |
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| 作者姓名： | 丁蕾 朱声荣 谢三祥 吴祥冰 |
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| 作者单位： | Department of Stomatology Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Stomatology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Stomatology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Stomatology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China |
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| 摘 要： | To observe the effects of basic fibroblast growth factor （bFGF） on human adenoid cystic carcinoma ACC-2 cell line proliferation and ERK, cyclin D1/p21^waf/cip1 signaling pathways, human adenoid cystic carcinoma cells （ACC-2） were cultured and the influence of bFGF of different concentrations on cell proliferation was determined by MTT. Protein was detected by immuno-precipitation and ERK activity by using ERK agent kit. p-ERK1/2 and down-stream cyclin D1, p21^waf/cip1 expression were detected by Western blotting and the interfering role of mitogen protein-activated kinase （MEK） suppressor U0126 in the afore-mentioned indicators was examined. MTT demonstrated ACC-2 cell proliferation was substantially enhanced by bFGF, immuo-precipitation displayed ERK activity was up-regulated by bFGF, and immuno-imprinting also showed p-ERK1/2, cyclin D1 expression was greatly enhanced and p21^waf/cip1 expression was inhibited by bFGE U0126 suppressed the effect of bFGF. It is concluded that bFGF can promote the proliferation of human adenoid cystic carcinoma ACC-2 cells, and its pathways are associated with the up-regulated activity and expression of p-ERK1/2, inhibited p21waf/cip1 expression and enhanced cyclin D1 expression.
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| 关 键 词： | 腺样囊性癌 纤维原细胞 淋巴组织 激酶 |
Effect of exogenous bFGF on the proliferation of human adenoid cystic carcinoma ACC-2 cells |
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| Lei Ding,Shengrong Zhu,Sanxiang Xie,Xiangbing Wu.Effect of Exogenous bFGF on the Proliferation of Human Adenoid Cystic Carcinoma ACC-2 Cells[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(2):227-229. |
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| Authors: | Lei Ding Shengrong Zhu Sanxiang Xie Xiangbing Wu |
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| Institution: | Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China |
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| Abstract: | To observe the effects of basic fibroblast growth factor(bFGF) on human adenoid cystic carcinoma ACC-2 cell line proliferation and ERK,cyclin D1/p21waf/cip1 signaling pathways,human adenoid cystic carcinoma cells(ACC-2) were cultured and the influence of bFGF of different con-centrations on cell proliferation was determined by MTT.Protein was detected by im-muno-precipitation and ERK activity by using ERK agent kit.p-ERK1/2 and down-stream cyclin D1,p21waf/cip1 expression were detected by Western blotting and the interfering role of mitogen pro-tein-activated kinase(MEK) suppressor U0126 in the afore-mentioned indicators was examined.MTT demonstrated ACC-2 cell proliferation was substantially enhanced by bFGF,im-muo-precipitation displayed ERK activity was up-regulated by bFGF,and immuno-imprinting also showed p-ERK1/2,cyclin D1 expression was greatly enhanced and p21waf/cip1 expression was inhibited by bFGF.U0126 suppressed the effect of bFGF.It is concluded that bFGF can promote the prolifera-tion of human adenoid cystic carcinoma ACC-2 cells,and its pathways are associated with the up-regulated activity and expression of p-ERK1/2,inhibited p21waf/cip1 expression and enhanced cyclin D1 expression. |
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| Keywords: | basic fibroblast growth factor adenoid cystic carcinoma extracellular signal-regulated kinase cell cycle phase protein D1 |
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