| 白头翁组织培养研究 |
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| 引用本文： | 张子学,丁为群,唐勇,时维静,叶文才. 白头翁组织培养研究[J]. 中国中药杂志, 2004, 29(3): 215-218 |
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| 作者姓名： | 张子学 丁为群 唐勇 时维静 叶文才 |
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| 作者单位： | 1. 安徽技术师范学院,安徽,凤阳,233100
2. 中国药科大学,江苏,南京,210009 |
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| 摘 要： | 目的 :为加快白头翁种苗生产速度及其生产的现代化。方法 :利用组织培养的方法 ,在添加不同种类植物激素处理组合的培养基上 ,对不同的外植体进行初代培养、丛生芽继代培养及其生根培养进行研究。结果 :适宜于白头翁的初代培养基 :MS + 6-BA 1.0～ 30mg·L -1+NAA 0～ 0.05mg·L -1+蔗糖 3 0g·L -1;增殖培养基 :MS + 6-BA 0 .2mg·L -1+NAA 0.02mg·L -1+BR 0.000 01mg·L -1+蔗糖 3 0g·L -1;生根培养基 :1/ 2MS +NAA 0.4mg·L -1+蔗糖 20g·L -1。结论 :采用白头翁的茎尖和花蕾作为外植体 ,利用组织培养的方法能够实现种苗的工厂化快速繁殖。
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| 关 键 词： | 白头翁 组织培养 快速繁殖 |
| 文章编号： | 1001-5302(2004)03-0215-04 |
| 收稿时间： | 2003-07-17 |
Study on tissue culture of pasqueflower |
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| ZHANG Zi-xue ;DING Wei-qun ;TANG Yong ;SHI Wei-jing ;YE W en-cai. Study on tissue culture of pasqueflower[J]. China Journal of Chinese Materia Medica, 2004, 29(3): 215-218 |
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| Authors: | ZHANG Zi-xue DING Wei-qun TANG Yong SHI Wei-jing YE W en-cai |
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| Affiliation: | Anhui Technical Teacher College, Fengyang 233100 Anhui, China. zzxue2003@yahoo.com.cn |
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| Abstract: | OBJECTIVE: To speed up seedling production of pasqueflower (Puzlsatilla chinenses) and their modernization in pasqueflower. METHOD: With tissue culture method, primary culture of different explants, culture of cluster buds and their rooting culture were conducted on medium of treatment combinations of adding different hormones. RESULT: The appropriate medium for different culture stages were MS + 6-BA 1.0-3.0 mg x L(-1) + NAA 0-0.05 mg x L(-1) + Sucrose 30 g x L(-1) in primary culture, MS + 6-BA 0.2 mg x L(-1) + NAA 0.02 mg x L(-1) + BR 0.00001 mg x L(-1) + Sucrose 30 g x L(-1) in differentiation and subculture of cluster buds, 1/2 MS + NAA 0.4 mg x L(-1) + Sucrose 20 g x L(-1) in rooting. CONCLUSION: Applying stem tip and flower buds as explants, high frequency propagation of seedlings can be achieved with plant tissue culture in Pasqueflower. |
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| Keywords: | pasqueflower tissue culture high freque ncy propagation |
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