黄独脱毒苗快繁技术的研究

目的 以黄独脱毒苗为试材,研究不同因素对黄独带芽茎段芽增殖和生根的影响,以期对黄独脱毒苗的快繁技术进行优化.方法 采用植物组织培养的方法 进行茎尖培养和快繁研究,采用RT-PCR法对茎尖脱毒植株进行病毒检测.结果 黄独脱毒苗带芽苇段的最佳培养基是MS+KT 2 mg/L+6-BA 1 mg/L+NAA 0.5 mg/L;黄独脱毒苗带芽茎段增殖的最佳蔗糖质量浓度和琼脂质量浓度分别是30和0 g/L;黄独脱毒苗带芽茎段生根的最佳培养基是1/2 MS+IBA 0.1 mg/L+NAA 0.5 mg/L+PP_(333) 1 mg/L;黄独试管苗移栽的最好基质是珍珠岩:蛭石(2:1);黄独试管苗移栽时最佳的PP_(333)质量浓度是50 mg/L.结论 首次成功建立了黄独脱毒苗的快繁技术,为黄独脱毒苗的工厂化生产奠定了技术基础.

Rapid propagation technology of Dioscorea bulbifera virus-free plantlets

Objective Effects of different factors on proliferation and rooting of stems with a bud were studied by using Dioscorea bulbifera as test material to optimize the rapid propagation system of D.bulbifera virus-free plantlets.Methods Plant tissue culture method was used in shoot tip culture and rapid propagation study,and RT-PCR method was used in virus detection of virus-free plantlets.Results The best proliferation medium of D.bulbifera stems with a bud was MS+KT 2 mg/L+6-BA 1 mg/L+NAA 0.5 mg/L;The best sucrose and agar concentration of D.bulbifera stems with a bud was 30 g/L and 0 g/L,respectively;The best rooting medium of D.bulbifera stems with a bud was 1/2 MS+IBA 0.1 mg/L+NAA 0.5 mg/L+PP_(333) 1 mg/L;The best transplanting matrix of regeneration plantlets from D.bulbifera stems with a bud was perlite-vermiculite(2:1);The best PP_(333) concentration of D.bulbifera regeneration plantlets for transplanting was 50 mg/L.Conclusion The rapid propagation system of D.bulbifera virus-free plantlets is established successfully for the first time,which provides a technological basis for factory production of D.bulbifera virus-free plantlets.