雌激素对视网膜血管渗漏的影响

目的探讨用去势方法研究雌激素影响眼部疾病的可行性以及雌激素对视网膜血管渗漏的影响。方法健康成熟的雌性SD大鼠22只，随机分为实验组和对照组。实验组雌性SD大鼠用去势方法降低体内雌激素水平；对照组用假去势方法，不影响雌激素水平。手术效果用阴道上皮涂片方法证实。用光动力诱导方法建立视网膜静脉阻塞模型，用分光光度计测量视网膜伊文思蓝渗漏量。结果实验组大鼠阴道上皮成熟值降低明显（t=21.008，P=0.000），对照组大鼠成熟值变化不明显（t=0.319，P=0.756）。实验组大鼠视网膜平均伊文思蓝渗漏量为（25.503 0±4.378 47）ng/mg，对照组大鼠视网膜平均伊文思蓝渗漏量为（17.830 0±4.265 69） ng/mg。2组之间差异有统计学意义（t=3.969 36，P=0.001）。结论去势方法是研究雌激素在眼部疾病作用的可靠模型，雌激素水平降低时视网膜血管的渗漏增加。(中华眼底病杂志,2005,21:174-176)

Effect of estrogen on the permeability of retinal blood vessel

ObjectiveTo explore the effect of estrogen on the permeability of retinal blood vessel by ovariectomy.MethodsTwenty-two healthy rats were divided into experimental and control group randomly. Estrogen level of rats decreased due to ovariectomy in the experimental group while stabilized by sham-ovariectomy in the control group. The results were confirmed by vaginal epithelium smearing. Retinal vein occlusion was established by photodynamic method, and leakage of Evan's blue in retina was determined by spectrophotometer.ResultsMature value of vaginal epithelium decreased significantly in ovariectomy rats（t=21.008，P=0.000） while not significantly in sham-ovariectomy ones （t=0.319，P=0.756）; the mean leakage of Evans blue was (25.503 0±4.378 47) ng/mg in experimental group, and (17.830 0±4.265 69) ng/mg in the control group, and the difference between the two groups is significant（t=3.969 36，P=0.001）.ConclusionOvariectomy is an useful method to study the effect of estrogen on ocular diseases, and when estrogen level decreases, the permeability of retinal blood vessel increases.(Chin J Ocul Fundus Dis, 2005,21:174-176)