利用腺病毒载体导入CTLA4Ig基因对大鼠同种心脏移植免疫抑制作用的研究

目的　探索CTLA4Ig基因在实验动物体内的表达以及对大鼠同种心脏移植后的免疫抑制作用。方法　利用腺病毒作载体 ,将CTLA4Ig基因导入同种心脏移植的受体鼠体内 ,以编码 β 半乳糖苷酶的有复制缺陷的腺病毒重组体 (Adex/LacZ)为对照 ,观察该基因在实验动物体内的表达以及对同种异体心脏移植后的免疫抑制作用。逆转录多聚酶链反应 (RT PCR)法检测CTLA4Ig基因在大鼠体内的表达 ,并用流式细胞仪来测定血中CTLA4Ig蛋白质的浓度变化。 结果　导入的CT LA4Ig基因能够在大鼠肝脏中表达 ,血中CTLA4Ig的浓度明显高于对照组 (P <0 .0 5 ) ,而且移植后心脏的存活时间显著长于对照组 (P <0 .0 1)。结论　CTLA4Ig基因能在大鼠体内良好表达并明显拮抗同种移植排斥反应

Immunoinhibitive effects of adenovirus-mediated transfer of CTLA4Ig gene on heart allograft in rats

Objectives To investigate the expression of CTLA4Ig gene and its immunoinhibitive effects on the heart allograft in rats.Methods The CTLA4Ig gene was cloned into adenovirus and introdueed into the experimental recipient rats during heart allograft.The replication-defective recombinant adenovirus encoding the Escherichia Coli Lac Z gene able to produce β-galaetosidase served as control.RT-PCR was employed to detect the expression of CTLA4Ig gene in the recipient rat and flow cytometer used to monitor the blood alteration of CTLA4Ig product concentration.Results In the experimental group,the introduced CTLA4Ig gene could be expressed highly in the recipient rat liver and the blood concentration of CTLA4Ig was significantly higher than in the control group(P＜0.05).The survival time of heart allograft in experimental group was obviously longer than in the control group(P＜0.01).Conclusions The CTLA41g gene could bewdl expressed in the recipient rats and obviously antagonize the rejection of the allografts.