DA与Lewis大鼠品系组合建立大鼠肝移植排斥与耐受模型

目的 建立大鼠原位肝脏移植急性排斥与自然耐受模型.方法 采用近交系雄性DA大鼠与Lewis大鼠互为供受体,改良"二袖套"法建立大鼠原位肝脏移植(rat orthotopic liver trans-plantation,ROLT)模型84例.实验分为4组:排斥组(DA→Lewis,n=12),FK506处理排斥组DA→Lewis,n=24,术后1～7 d用FK506 0.2 mg/(kg·d)灌胃],耐受组(Lewis→DA,n=24),同基因组(DA→DA,n=24).各组中随机取6例观察生存期,其余分别于术后7、14、28 d处死6例,收集外周血及肝脏标本.检测血清标本天冬氨酸转氨酶(aspartate aminotransferase,AST)、总胆红素(total bilirubin,TBIL)浓度,病理学检查移植物排斥反应程度.结果 排斥组中位生存时间(median surviv-al time,MST)为12 d,而FK506处理排斥组MST为76 d,较排斥组明显延长(vs排斥组,P<0.01).耐受组与同基因组的MST均>120 d.术后7 d,排斥组血清AST、BILI浓度均明显高于其余3组(P<0.05);术后14 d,FK506处理组、耐受组和同基因组血清AST、TBIL浓度无明显差异.术后28 d,FK506处理组血清AST、TBIL浓度较耐受组和同基因组明显升高(P

Establishment of rejection and tolerance model of rat orthotopic liver transplantation by DA and Lewis strain combination

Objective To establish rejection and tolerance model of rat orthotopic liver transplantation(ROLT).Methods Eighty-four cases of ROLT with inbred male DA and Lewis rats serving as donors and recipients respectively, undergoing modified Kamada two-cuff technique were divided into 4 groups: rejection group(DA→Lewis,n=12),rejection group treated with FK506(DA→group(Lewis→DA,n=24),isogenic group(DA→DA,n=24).After transplantation,6 recipients sacrificed one day 7,14 and 28,respectively.The serum AST and bilirubin levels and graft histological changes were evaluated. Results The median survival time(MST)of rejection group was shorter than FK506 treatment group(1 2 d vs 76 d,P<0.01)and the MST in tolerance and isogenic group was more than 120 d. In 7 d after transplantation, the serum concentrations of AST and TBIL in reiecrion group were significantly higher than those in the other three groups(P