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| RP-HPLC法同时测定金银花中的2种三萜皂苷 | |
| 引用本文： | 张小光,王智民,宋小妹,朱晶晶,高慧敏,林丽美.RP-HPLC法同时测定金银花中的2种三萜皂苷[J].中国中药杂志,2007,32(20):2149-2151. |
| 作者姓名： | 张小光 王智民 宋小妹 朱晶晶 高慧敏 林丽美 |
| 作者单位： | 1. 中国中医科学院,中药研究所,北京,100700;陕西中医学院,陕西,咸阳,712046 2. 中国中医科学院,中药研究所,北京,100700 3. 陕西中医学院,陕西,咸阳,712046 |
| 摘 要： | 目的：建立同时测定金银花中三萜皂苷成分忍冬苦苷A和忍冬苦苷C含量的方法。方法：采用反相高效液相色谱法,Alltech C18柱(4.6 mm×250 mm,5 μm),流动相甲醇-乙腈-0.1%磷酸,检测波长210 nm。结果：忍冬苦苷A和忍冬苦苷C分别在0.15～2.25 μg(r=0.999 9)和0.11～1.65 μg(r=0.999 1),呈现良好线性关系,平均加样回收率分别为99.9%,98.3%。结论：忍冬苦苷A和忍冬苦苷C在不同地区的金银花药材中含量差异较大。 |
| 关 键 词： | 金银花 忍冬苦苷A 忍冬苦苷C |
| 文章编号： | 1001-5302（2007）20-2149-03 |
| 收稿时间： | 1/3/2007 12:00:00 AM |
| 修稿时间： | 2007-01-03 |
Determination of loniceroside A and C in Lonicerae japonicae by RP-HPLC |
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| ZHANG Xiao-guang;; WANG Zhi-min; SONG Xiao-mei; ZHU Jing-jing; GAO Hui-min; LIN Li-mei.Determination of loniceroside A and C in Lonicerae japonicae by RP-HPLC[J].China Journal of Chinese Materia Medica,2007,32(20):2149-2151. | |
| Authors: | ZHANG Xiao-guang;; WANG Zhi-min; SONG Xiao-mei; ZHU Jing-jing; GAO Hui-min; LIN Li-mei |
| Institution: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. |
| Abstract: | OBJECTIVE: To develop an HPLC method for determination of loniceroside A and loniceroside C in Lonicerae japonicae. METHOD: The analysis was carried out on an Alltech C18 column (4.6 mm x 250 mm, 5 microm) evaluated with methanol-acetonitrile-0.1% glacial acetic acid as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 210 nm for UV detection. RESULT: The calibration curves were linear over the range from 0.15 to 2.25 microg (r = 0.999 9) for loniceroside A and 0.11 to 1.65 microg (r = 0.999 1) for loniceroside C. The average recoveries were 99.9% and 98.3%, respectively. CONCLUSION: The contents of Loniceroside A&C are diverse in Flos Lonicerae japonicae in different regions. |
| Keywords: | RP-HPLC |
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