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| 苯乙酸对胶质瘤C6细胞p16基因的激活作用及意义 | |
| 引用本文： | 陈阳,陈宝琴,田宇. 苯乙酸对胶质瘤C6细胞p16基因的激活作用及意义[J]. 中国实验诊断学, 2002, 6(1): 16-17 |
| 作者姓名： | 陈阳 陈宝琴 田宇 |
| 作者单位： | 1. 天津石化医院外二科,天津,300270 2. 吉林省人民医院,吉林,长春,130021 3. 吉林大学中日联谊医院神经外科,吉林,长春,130021 |
| 基金项目： | 国家自然科学基金资助项目 ( 3990 0 15 2 ) |
| 摘 要： | 目的 观察诱导分化剂苯乙酸对C6胶质瘤细胞增殖分化过程中抑癌基因p16的蛋白水平表达变化的影响。方法 3 H -TdR掺入法测细胞增殖率 ;免疫组化SP法检测p16基因蛋白水平的表达。结果 应用苯乙酸后 ,细胞cpm值降低 ;p16基因胞浆阳性表达显著升高。结论 苯乙酸对胶质瘤细胞存在剂量依赖性抑制 ,增强了抑癌基因p16表达活性 ,促使C6细胞向凋亡方向转化 |
| 关 键 词： | 苯乙酸 胶质瘤 p16基因 |
| 文章编号： | 1007-4287(2002)06-0016-02 |
| 修稿时间： | 2001-05-25 |
Growth- inhibitory and ditferentiation- inducing activity of phenylacetate and increased effect of p16 gene on glioma cell C6 |
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| CHEN Yang,CHEN Bao-qin,TIAN Yu,et al,. Growth- inhibitory and ditferentiation- inducing activity of phenylacetate and increased effect of p16 gene on glioma cell C6[J]. Chinese Journal of Laboratory Diagnosis, 2002, 6(1): 16-17 | |
| Authors: | CHEN Yang CHEN Bao-qin TIAN Yu et al |
| Abstract: | Abstrait:Objective To determine the growth-inhibitory and differentiation-induing activity of phenylacetate (PA)on glioma cell line C6,to find the change of p16 gene expression.Methods The effect of PA on cell proliferation using 3 H-TdR assay, P16 gene protein expression studied by immunohistochemistry technique.Results At does ranges 0.625?2 5?5 0 nmol/L,PA caused a dose dependent proliferation inhibitory effect. The protein expression of pl6 gene was notably increased by PA.Conclusion PA caused a does-edpendent proliferation inhibitory effect,the expression of p16 gene activity increased by PA. |
| Keywords: | phenylacetate glioma p16gene |
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