| 不同长度疟原虫环子孢子蛋白重复序列DNA免疫的实验分析 |
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| 引用本文： | 孙明,王炯,董承红,王丽春,李琦涵. 不同长度疟原虫环子孢子蛋白重复序列DNA免疫的实验分析[J]. 现代免疫学, 2000, 20(6): 326-328 |
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| 作者姓名： | 孙明 王炯 董承红 王丽春 李琦涵 |
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| 作者单位： | 中国医学科学院,中国协和医科大学医学生物学研究所,昆明,650118 |
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| 摘 要： | 本实验利用DNA免疫技术 ,将PCR得到的疟原虫环子孢子蛋白 (CSP )不同长度的重复序列基因克隆到pcDNA3质粒中 ,对小鼠进行肌肉注射 ,经过免疫检测 ,结果证明不同长度CSP的重复序列能够单独诱发机体全面的细胞免疫和体液免疫 ,这为今后疟疾疫苗的发展提供了新的思路。
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| 关 键 词： | DNA免疫 CSP基因重复序列 细胞毒T细胞杀伤实验 |
| 文章编号： | 1001-2478(2000)06-0326-03 |
| 修稿时间： | 2000-02-22 |
A Preliminary Analysis on the DNA Vaccination with Different Sizes of Repeat Sequences of Circumnsporozoite Protein Gene |
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| Sun Ming,Wang Jiong,Dong Chenghong,Wang Lichun,Li Qihang. A Preliminary Analysis on the DNA Vaccination with Different Sizes of Repeat Sequences of Circumnsporozoite Protein Gene[J]. Current Immunology, 2000, 20(6): 326-328 |
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| Authors: | Sun Ming Wang Jiong Dong Chenghong Wang Lichun Li Qihang |
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| Affiliation: | Sun Ming,Wang Jiong,Dong Chenghong,Wang Lichun,Li Qihang;(Institute of Medical Biology, CAMS & PUMC,Kuming 650118) |
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| Abstract: | The different sizes of repeat sequences encoding the CSP of Plasmodium falciparium were cloned into plasmid pcDNA3 and BALB/c mice were inoculated intramuscularly with this recombinant plasmid DNA It was found that the vaccination with this plasmid DNA could elicite both humoral and cellular immune responses These results privide a new way of approaches to the reseach of human malaria vaccines |
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| Keywords: | DNA immunology different size repeat sequences of CSP gene CTL |
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