银屑病患者生长抑素的定量定位检测

目的 探讨生长抑素(somatostatin,SS)与银屑病的相关性.方法 用放射免疫分析法测定银屑病患者血浆及皮损中SS含量,用免疫组化法观察13例寻常型银屑病患者皮损和8例正常人皮肤中SS的分布,用原位杂交组织化学法检测皮损处SSmRNA表达情况.结果 定量检测表明患者血浆SS水平低于对照组,而患者皮损SS水平显着高于对照组,定位检测光镜下见3例新发银屑病患者皮损中的SS在真皮乳头层大片沉积,而10例慢性急性发作的病例与正常对照基本相同,光镜下见真皮层极散在分布的SS染色.对3例皮损中SS强阳性的银屑病患者,用原位杂交组织化学法检测皮损处SSmRNA表达情况,结果未见皮肤各层细胞内有SSmRNA表达信号.结论 银屑病患者SS血浆中的低水平及其皮损中的高表达提示SS在银屑病的发病中起重要作用.

Quantitation and Localization of Somatostatin in Patients with Psoriasis

Objective To investigate the relationship between somatostatin and psoriasis. Methods The levels of somatostatin in the plasma and skin lesions of patients with psoriasis were determined by radioimmunoassay. The distribution of somatostatin in the skin lesions of 13 patients and eight normal skin samples was determined by ABC alkaline phosphatase immunohistochemical assay. The somatostatin mRNA expression was examined by in situ hybridization histochemistry. Results The plasma level of somatostatin of the patients was significantly lower than that in controls (P< 0.05), whereas the concentration of somatostatin in the skin lesions was significantly higher than that in controls (P< 0.05). The distribution of somatostatin was accumulated in patches in the papillary layer in three incipient cases. In 10 chronic cases with acute onset the distribution was the same as the normal controls, and scattered staining of somatostatin could be seen in the dermis. The three cases with strong positive staining of somatostatin were tested for the expression of somatostatin mRNA by in situ hybridization histochemistry. No signal of somatostatin mRNA expression was observed in any cells of the skin lesions. Conclusion The lower level of somatostatin in the plasma and high expression of somatostatin in the skin lesions suggest that somatostatin might play an important role in the pathogenesis of psoriasis.