葡萄糖酸镁对大鼠心肌缺血再灌注损伤的保护作用

目的:观察葡萄糖酸镁对心肌缺血再灌注损伤的影响,探讨葡萄糖酸镁心肌保护作用及其机理。方法:采用改进了的整体大鼠急性心肌缺血再灌注损伤模型,检测组织内Na+- K+- ATPase活力及丙二醛(MDA)和Ca2 +含量,并测定由ADP诱导的血小板5min最大聚集率AGG (M )。结果:与假手术组相比,缺血再灌注组心肌组织内Na+-K+ -ATPase活力显著下降(P <0 .0 1) ,MDA和Ca2 +含量明显升高(P<0 .0 1) ,AGG(M)升高(P <0 .0 5 )。与缺血再灌注组比较,葡萄糖酸镁3个不同剂量保护组心肌组织内Na+ -K+ -ATPase活力明显升高(P <0 .0 1) ,MDA和Ca2 +含量明显降低(P <0 .0 5或P <0 .0 1) ,AGG(M)也明显降低(P <0 .0 1) ,且各作用均随用药剂量的增加而更加显著。结论:葡萄糖酸镁对心肌缺血再灌注损伤有保护作用,其保护作用机制与增加Na+ -K+- ATPase活性、减轻钙超载和抑制脂质过氧化反应以及血小板聚集有关。

Cardioprotective effects of magnesium gluconate on myocardial ischemia reperfusion injury in rats

AIM: To observe the effects of magnesium gluconate on myocardial ischemia reperfusion injury in anesthetized rats and to investigate the cardioprotective effects of magnesium gluconate. METHODS: The improved model of myocardial ischemia reperfusion injury in anesthetized rats was adopted. Na +-K +-ATPase activity, malondialdehyde (MDA) and Ca 2+ contents in myocardium were assayed. The platelet aggregation AGG(M)and Mg 2+ in blood serum were assayed at the same time. RESULTS: Na +-K +-ATPase activity decreased and MDA and Ca 2+ contents increased in the ischemia reperfusion group compared with sham. The AGG(M)and Na +-K +-ATPase activity increased, and MDA and Ca 2+ contents and AGG(M)decreased in the three different dosages group of magnesium gluconate compared with the ischemia reperfusion group. The cardioprotective effects increased with the dose increasing of magnesium gluconate. CONCLUSION: Magnesium gluconate has protective effects on myocardial ischemia reperfusion injury in rats. It may be related to increasing the activity of Na +-K +-ATPase, and to relieving calcium overload and inhibiting lipid peroxidation and platelet aggregation.