慢性阻塞性肺疾病患者血清中MMP-9和TNF-α的检测及其相关研究

目的研究慢性阻塞性肺疾病（COPD）患者急性加重期和稳定期血清中MMP-9和TNF-α变化的临床意义。方法采用ELISA方法，分别检测25例患者在COPD急性加重期、稳定期及20例正常人血清MMP-9和TNF-α水平，并进行比较。结果COPD急性加重期TNF-α水平高于稳定期、正常人，而后两组TNF-α水平相近；但三组MMP-9水平相近。结论TNF-α参与了COPD的炎症过程，其血清水平有助于对COPD急性加重期进行检测。MMP-9在COPD急性加重期和稳定期都有高水平表达，尚待进一步观测。

Serum levels of TNF-α(e)and MMP-9 in patients with chronic obstructive pulmonary disease and its clinical significance

Objective To study the variation and significance of TNF-α and MMP-9 in patients with chronic obstructive pulmonary disease （COPD） during acute exacerbation and stable periods. Methods Enzyme-llnked immunosorbentassay was used to measure the serum levels pf TNF-α and MMP-9 in 20 normal subjects, 25 patients with COPD during acute exacerbation and stable periods. Results The serum level of TNF-α in patients with COPD during acute exacerbation period was significantly higher than that of stable period （P〈0.01）. The serum level of MMP-9 did not differ significantly in acute exacerbation period as compared with stable period （P〈0. 05）, but the serum levels of MMP-9 in acute exacerbation and stable periods were both significantly higher than those of normal persons （P〈0. 01）. Conclusion TNF-α takes part in the inflammation activity. Its serum level is helpful to determine the condition of COPD during acute exacerbation period. The serum level of MMP-9 in patients with COPD is higher, suggesting MMP- 9 may be involved in the airway remodeling and pulmonary emphysema development.