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| 大鼠脑出血后行为学改变和室管膜下区细胞增殖规律 | |
| 引用本文： | 孙建军,刘勇,张蓬勃,陈新林,郭振宇,张建水,杨蓬勃.大鼠脑出血后行为学改变和室管膜下区细胞增殖规律[J].中南大学学报(医学版),2009,34(3):236-241. |
| 作者姓名： | 孙建军 刘勇 张蓬勃 陈新林 郭振宇 张建水 杨蓬勃 |
| 作者单位： | 西安交通大学医学院1.第二附属医院神经外科， 西安 710004； 2. 神经生物研究所， 西安 710061；3. 第二附属医院麻醉科， 西安 710004 |
| 摘 要： | 目的：研究成年大鼠脑出血（intracerebral hemorrhage,ICH）后行为学改变和室管膜下区（subventricular zone，SVZ）神经前体细胞增殖的关系。方法：40只雄性SD大鼠随机分为行为学检测组 (n=19)和Bromodeoxyuridine (Brdu)免疫组织化学染色组(n= 21)。立体定向注射Ⅶ型胶原酶建立大鼠纹状体ICH模型。脉冲法腹腔注射Brdu标记增殖细胞。在ICH后第2,7,14及28天处死大鼠，分别行行为学检测和Brdu免疫组织化学染色，行为学评分采用前肢放置实验、Berderson评分法及角落转向实验；对SVZ Brdu免疫阳性细胞作细胞计数。结果：ICH后第2天大鼠出现严重的神经功能障碍，其神经功能在4周内逐渐恢复；大鼠ICH后第2天双侧SVZ Brdu阳性细胞数开始增加，7 d时达高峰，14 d仍可见较多的增殖细胞，28 d时Brdu阳性细胞数降至对照水平。结论：大鼠ICH后神经功能与SVZ细胞增殖存在时间上的相关性，提示SVZ细胞可能参与ICH后组织修复过程。 |
| 关 键 词： | 脑出血 行为学 室管膜下区 增殖 |
| 收稿时间： | 2008-2-23 |
Behavioral change and cell proliferation in the subventricular zone in adult rats after intracerebral hemorrhage |
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| SUN Jianjun,LIU Yong,ZHANG Pengbo,CHEN Xinlin,GUO Zhenyu,ZHANG Jianshui,YANG Pengbo.Behavioral change and cell proliferation in the subventricular zone in adult rats after intracerebral hemorrhage[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),2009,34(3):236-241. | |
| Authors: | SUN Jianjun LIU Yong ZHANG Pengbo CHEN Xinlin GUO Zhenyu ZHANG Jianshui YANG Pengbo |
| Institution: | 1.Department of Neurosurgery, Second Affiliated Hospital of Medical School, Xi’an Jiaotong University, Xi’an 710004; 2.Institute of Neurobiology, Medical School of Xi’an Jiaotong University, Xi’an 710061； 3.Department of Anaesthesia, Second Affiliated Hospital of Medical School, Xi’an Jiaotong University, Xi’an 710004, China |
| Abstract: | ObjectiveTo investigate the relationship between behavioral changes and cell proliferation in subventricular zone (SVZ) after intracerebral hemorrhage (ICH) in adult rats.MethodsForty male Sprague-Dawley rats were randomly assigned into a behavioral test group (n=19) and a bromodeoxyuridine (Brdu) immunohistochemical staining group (n= 21).ICH was induced by stereotactial injection of collagenase typeⅦ into straitum. Proliferating cells were labeled by injection intrapenitoneally of bromodeoxyuridine in a pulse protocal. Rats were killed on day 2,7,14, and 28 after the ICH.Behavioral test and bromodeoxyuridine immunohistochemical staining were performed.Behavioral change was tested by forelimb placing test, Berderson’s grade and corner turn test in rats. Cell counting of bromodeoxyuridine immunoreactive cells in SVZ was performed. ResultsThere were marked neurological deficits by day 2 after the ICH, with progressive recovery of function over 4 weeks. A significant increase in the number of bromodeoxyuridine immunoreactive cells in the ipsilateral and cortralateral SVZ was observed from 2 to 14 days with a peak at day 7 after the ICH compared with the sham group.The bromodeoxyuridine immunoreactive cells decreased to control level 28 days after the ICH. ConclusionProliferation of cells in SVZ corresponds well with behavioral recovery after the ICH, which indicates SVZ cells may be involved in the repairing process after the ICH. |
| Keywords: | intracerebral hemorrhage behavior subventricular zone proliferation |
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