长期酒精摄入对大鼠糖酵解途径关键酶活性的影响

目的　研究长期酒精摄入对大鼠糖酵解途径及其关键酶活性的影响。方法　 70只Wistar大鼠 (雌雄各半 )随机分为 5组 ,分别给予蒸馏水或不同浓度的酒精溶液 (10 %、30 %、5 0 % ,V V)灌胃。 9周后 ,取肝脏测定糖酵解途径关键酶 -葡萄糖激酶 (GK)、磷酸果糖激酶 (PFK)和己糖激酶 (HK)的活性。结果　 (1) 30 %、5 0 %酒精组大鼠GK和PEK活性与对照组相比降低 (P <0 0 5 ) ;(2 )各组大鼠HK活性差异均没有显著性 ;(3)GK、PFK活性与酒精剂量显著相关 (r =- 0 99～ - 0 97,P <0 0 5 )。结论　长期酒精摄入可使GK和PFK)活性降低 ,从而抑制糖酵解过程。这也可能是酒精升高血糖 ,影响血糖调节机制进而导致糖尿病的机制之一

Effects of chronic alcohol administration on the activities of the key glycolytic enzymes of rats

In order to study the effects of chronic alcohol administration on the activities of the key glycolytic enzymes, a oral administration of alcohol solution in different concentrations was given to rats for 9 weeks. The activities of glucokinase (GK), phosphofructokinase (PFK) and hexokinase (HK) in liver were determined at the end of the study. The results showed that the activities of GK and PFK in the groups administrated with 30% and 50% alcohol solution decreased obviously in comparison with that in control group (P < 0.05). But the activity of HK had no significant differences in all five groups. Correlation analysis indicated that the activities of GK and PFK were correlated with the dose of alcohol significantly (r = -0.99(-)-0.97, P < 0.05). These results demonstrated that chronic alcohol administration can suppress the glycolytic pathway. They can also be one of the mechanisms of diabetes mellitus relating to alcohol because of the high blood sugar and the disturbed blood sugar regulation.