针刺对小鼠巨噬细胞基因表达的效应

目的 为了探讨针刺镇痛效应与细胞免疫的关系，研究了针刺对小鼠巨噬细胞中ｃ－ｆｏｓ ｍＲＡＮ ｐｐＥＮＫｍＲＮＡ，ｉＮＯＳｍＲＮＡ及ｉＮＯＳ活性的效应。方法 ２０只ＢＡＬＢ／ｃ小鼠被随机分成２组；ａ．用５Ｈｚ电针处理的针刺组；ｂ．未用电针处理的对照组。针刺或牵拉前后用钾离子渗透法检测痛阈。实验采用原位杂交，ＮＡＤＰＨＮＢＴ组织化学，ＲＮＡ斑点印迹及蛋白质斑点印迹技术。应用ＴＬＣ扫描仪扫描斑点印迹信号并作统计学处理。结果 杂交信号和ｉＮＯＳ活性呈现蓝紫色，信号均定位于巨噬细胞的胞质。与对照组相比，针刺组的所有印迹信号均增强，Ｐ〈０．０１。针刺组中ｃ－ｆｏｓ ｍＲＮＡ，ｐｐＥＮＫｍＲＮＡ，ｉＮＯＳｍＲＮＡ的表达及ｉＮＯＳ的活性与提高的痛阈呈正相关。此外，ｃ－ｆｏｓ ｍＲＮＡ与ｍＲＮＡ与ｐｐＥＮＫｍＲＮＡ之间的变动呈正

ACUPUNCTURE EFFECT ON GENE EXPRESSION OF THE MOUSE PERITONEAL MACROPHAGE

Objective To investigate the relationship between acupuncture analgesia and cellular immunity, the acupuncture effect on expression of c fos mRNA, ppENKmRNA,iNOSmRNA and iNOS activity in the mouse peritoneal macrophage were studied. Methods Twenty BALB/c mice were randomly divided into 2 groups:a,the acupuncture group treated with 5Hz electroacupuncture (EA); b, the control group treated with no EA. The pain threshold was detected by K + ionophoresis method before and after EA or restraining. The experiment was performed by using in situ hybridization, NADPH NBT histochemistry, RNA dot blot and protein dot blot techniques. All dot blot signals were scanned for statistical analysis. Results The hybridization signals and iNOS activity appeared as granules in bluish violet color, localized in the cytoplasm of the macrophage. All dot blot signals were increased in the acupuncture group, when compared with those in the control group( P <0 01). Besides, there was a positive correlation in alteration between the analgesic effect and all the signals, and between the c fos mRNA signals and ppENKmRNA signals. Conclusion Acupuncture could induce analgesic effect and gene expression of c fos mRNA, ppENKmRNA and iNOS with positive correlation in the peritoneal macrophage, suggesting that acupuncture could promote cellular immunity through neuroimmunological pathway. The positive correlation in alteration between the c fos mRNA signals and ppENKmRNA signals with similar intensity suggests that the c fos may involve gene expression of ppENK and both genes may express about at the same time in the macrophage.