胃癌细胞固有荧光特征初探

目的:探讨正常胃上皮细胞和不同分化程度胃癌细胞的细胞内固有荧光特征性生物物质来源。方法:通过激光共聚焦显微镜比较2种不同分化程度的胃癌细胞株和正常胃上皮细胞株在波长500～530nm的固有荧光表现;以罗丹明123(Rodamine123)对细胞内线粒体进行染色,观察细胞固有荧光的位置分布;采用氧化呼吸链阻断剂羰基氰化物间氯苯腙(CCCP)阻断细胞氧化呼吸反应,测定细胞内固有荧光强度变化。结果:波长500～530nm的细胞固有荧光主要来自于细胞线粒体内,不同分化程度的胃癌细胞株荧光强度都明显弱于正常上皮细胞株。CCCP作用于细胞可提高该波长范围细胞内的荧光强度。结论:细胞在500～530nm波段固有荧光减弱的特征可能有助于诊断胃癌,该固有荧光的生物物质来源可能是参与线粒体内氧化呼吸作用的重要辅酶还原型烟酰胺腺嘌呤二核苷酸(NADH)。

Preliminary study on characteristic autofluorescence in gastric cancer cells

Objective To investigate the biological origin of eharaeteristie autofluoreseenee in normal gastric epithelial cells and different degree differentiated gastric cancer cells. Methods The behaviors of autofluoreseenee at wavelength 500-530 nm between gastrie eaneer cells and normal gastric epithelial cells were compared by laser confoeal fluoreseenee microscopy. The site of autofluoreseenee was examined after stained by Rodamine 123. Alteration of autofluoreseence intensity was assessed after the addition of oxidative respiratory chain blocking agent earbonylcyanide-mehlorophenylhydrazone （CCCP）. Results Autofluoreseenee at wavelength 500-530 nm mainly came from mitoehondria. The autofluoreseenee intensity of all the different degree differentiated gastric eaneer cells was weaker than that of the normal gastric epithelial cells. The autofluoreseenee intensity increased with the addition of CCCP. Conclusions The weakening of autofluoreseenee at wavelength 500-530nm might help the diagnosis of gastrie cancer. The origin of the autofluoreseenee was probably the redox eoenzyme（reduced nieotinamide adenine dinueleotide, NADH） in mitochondria.