分子量对海参岩藻聚糖硫酸酯在体内吸收的影响

目的 利用高温高压降解法制备两种不同分子量的岩藻聚糖硫酸酯,探究口服不同分子量的海参岩藻聚糖硫酸酯的吸收特性。方法 采用分子排阻凝胶色谱法、离子高效液相色谱法,检测海参硫酸多糖高温高压降解前后分子量、硫酸根含量的变化,并利用PMP柱前衍生-高效液相色谱法测定岩藻聚糖硫酸酯的单糖组成,以及大鼠血清中单糖的变化。结果 口服低分子量海参岩藻聚糖硫酸酯后,大鼠血清中岩藻糖和半乳糖的吸收速度和最大浓度明显高于中分子量岩藻聚糖组,血清中甘露糖、氨基葡萄糖的含量也显著上升,血清中氨基半乳糖的含量略有上升。而口服中、低分子量岩藻聚糖硫酸酯都能够降低血清中葡萄糖的含量。结论 分子量低于10 kDa的低分子量岩藻聚糖具有很好的体内的吸收率,适合于开发口服岩藻聚糖功能产品。

Effect of molecular weight on absorption and metabolism of sea cucumber fucoidan

Objective To investigate the influence of molecular weight of fucoidan from sea cucumber on the absorption and monosaccharide composition in serum in vivo, two fucoidan with different molecular weight from sea cucumber were prepared by hydrothermal degradation. Methods High performance molecular exclusion gel chromatography, high performance anion-exchange chromatography were used to analyze the molecular weight, sulfate content and monosaccharide compositionof fucoidan, the monosaccharide composition and the absorption of fucoidan in rat serum were determined by PMP pre-column derivatization-high performance liquid chromatography. Results After oral administration of low molecular weight fucoidan, the absorption rate and concentration of fucose and galactose in rat serum were significantly higher than those in medium molecular weight group. At the same time, oral administration of low molecular weight fucoidan can significantly increase the content of mannose and glucosamine in serum and slightly increase the content of galactosamine in serum. Oral administration of medium and low molecular weight sea cucumber fucoidan can reduce the content of glucose in the serum. Conclusion The low molecular weight fucoidan with molecular weight lower than 10 kDa,was much more easy to be absorbed and is a good choice for the oral fuctional drug.