| 刀豆蛋白A促进T淋巴细胞增殖与ClC-3蛋白的关系 |
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| 引用本文： | 钱艳,关永源,王冠蕾,杨晓茹,贺华,丘钦英.刀豆蛋白A促进T淋巴细胞增殖与ClC-3蛋白的关系[J].中国药理学通报,2003,19(10):1132-1135. |
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| 作者姓名： | 钱艳 关永源 王冠蕾 杨晓茹 贺华 丘钦英 |
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| 作者单位： | 中山大学中山医学院分子医学研究中心,药理学教研室,广州,510089 |
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| 基金项目： | 广东省自然科学基金团队项目 (2 0 0 1)，中华医学基金会基金(NoCMB0 0 73 0 )，博士点基金资助 |
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| 摘 要： | 目的 研究刀豆蛋白A(ConA)促进T淋巴细胞增殖与内源性ClC 3蛋白的关系。方法 免疫印迹 (Westernblot)法检测ClC 3蛋白表达 ;3 H] TdR参入法观察ConA对细胞增殖的影响。结果 ①T淋巴细胞上有内源性ClC 3蛋白表达 ,分子量约为 80ku ;②ConA可浓度依赖性地促进T淋巴细胞增殖 ;③ConA刺激T淋巴细胞 2 4、4 8、72h后 ,ClC 3蛋白表达均增加 ;刺激 4 8h后ClC 3蛋白表达增加最多。④ConA可浓度依赖性地增加ClC 3蛋白表达。结论 T淋巴细胞上存在内源性ClC 3蛋白表达 ;ClC 3蛋白可能参与了ConA诱导的T淋巴细胞增殖过程
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| 关 键 词： | Cl-通道 ClC-3 T淋巴细胞 增殖 免疫印迹 |
| 文章编号： | 1001-1978(2003)10-1132-04 |
| 修稿时间： | 2003年4月30日 |
Relationship between ConA-induced T lymphocyte proliferation and ClC-3 protein |
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| QIAN Yan,GUAN Yong Yuan,WANG Guan Lei,YANG Xiao Ru,HE Hua,QIU Qin Ying.Relationship between ConA-induced T lymphocyte proliferation and ClC-3 protein[J].Chinese Pharmacological Bulletin,2003,19(10):1132-1135. |
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| Authors: | QIAN Yan GUAN Yong Yuan WANG Guan Lei YANG Xiao Ru HE Hua QIU Qin Ying |
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| Abstract: | AIM To study the expression of ClC 3 protein in human T lymphocyte and its relation with ConA induced T lymphocyte proliferation. METHODS With western blot, the expression of ClC 3 protein was detected, and through thymidine incorporation, the effects of ConA on human T lymphocyte were measured. RESULTS ①ClC 3 protein was endogenously expressed in human T lymphocyte;②ConA stimulated T lymphocyte proliferation in an dose dependent manner;③ConA enhanced ClC 3 protein expression in dose dependent manner; ④ClC 3 protein expression was all increased after incubating with ConA for 24,48,72 h with peak at 48 h. CONCLUSION ClC 3 protein is endogenously expressed in human T lymphocyte; it may take part in the proliferation of human T lymphocyte. |
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| Keywords: | chloride channel ClC 3 T lymphocytes proliferation western blot |
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