膀胱良、恶性上皮肿瘤中 MMP -9 的表达及临床意义简

目的：探讨MMP-9在正常膀胱组织和膀胱良、恶性肿瘤中的表达和临床意义。方法：采用免疫组织化学sP法检测15例正常膀胱黏膜、20例内翻性乳头状瘤、100例膀胱癌（包括86例膀胱尿路上皮癌、9例膀胱腺癌和5例膀胱鳞状细胞癌）中MMP-9的表达，观察其在膀胱良、恶性肿瘤中表达的差异，分析MMP-9表达与膀胱癌病理类型及临床病理特征的关系。结果：膀胱癌中MMP-9的阳性表达率为83．00％（83／100），显著高于正常膀胱移行上皮0（0／15）及内翻性乳头状瘤35．00％（7／20）两组（P〈0．05）。MMP-9的阳性表达率随浸润深度的增加及淋巴结的转移而升高（P〈0．05）。MMP-9在膀胱尿路上皮癌中的阳性表达率较膀胱腺癌及鳞状细胞癌为高，但差异无统计学意义（P〉0．05）。结论：MMP-9在膀胱癌组织中高表达，与膀胱癌的浸润及转移密切相关，其可能在膀胱恶性上皮肿瘤的发生发展过程中发挥重要作用，并可作为预后评估的重要指标之一。

Expression of MMP -9 in benign and malignant epithelial tumor of bladder and its clinical significance

Objective：To explore the expression of MMP -9 in benign and malignant epithelial tumor of bladder and its clinical significance. Methods ：The immunohistochemical staining assay was conducted to detect expression of MMP -9 in 15 cases of normal baldder epithelium,20 cases of bladder inverted papilloma and 100 cases of bladder carcinoma,including 86 cases of uroepithelium carcinoma of bladder （ BUC ） , 5 cases of squamous cell carcinoma of bladder and 9 cases of adenocarcinoma of bladder. Results：The positive rate of expression of MMP -9 in BUC 83.00% （83/100）was obviously higher than the normal tissue group 0 （0/15） and inverted papilloma group 35.00% （7/20） （ P 〈 0.05 ）. The positive rate of expression of MMP - 9 in high - grade BUC was significantly higher than in low - grade group. With the improvement of invasion tendency of BUC, the staining intensity of MMP - 9 was gradually increased（ P 〈 0.05 ）. Higher expression rate of MMP -9 was found in the BUC than in squamous cell carcinoma and adenoearcinoma, but there was no obvious difference （P 〉 0.05 ）. Conclusion： MMP- 9 was highly expressed in bladder carcinoma, which was closely associated with the invasion and metastasis of bladder carcinoma. It may play a very important role in the occurrence and progression of BUC and become a potential factor for evaluating prognosis of BUC.