EphB/Ephrin-B1信号对睫状神经节α7-尼古丁乙酰胆碱受体电流的影响

目的 探讨EphB/Ephrin.B1信号对鸡胚睫状神经节(CG)神经元上α7-尼古丁乙酰胆碱受体(α7-nAChRs)电流的影响. 方法用膜片钳技术分别在培养的和急性分离的CG神经元上记录α7-nAChRs电流.细胞被随机分为:对照组、Ephrin-BlFc处理组、IgG处理组和Ephrin-B1处理组,观察电流的变化. 结果对照组、lgG组和Ephrin-B1处理组之间α7-nAChRs电流无显著差异(P>0.05),而这3组与Ephrin-BlFc处理组之间有显著差异(P<0.05).Ephrin-BlFc可浓度依赖性抑制α7-nAChRs电流,该过程可在数分钟内发生,并呈现可逆性.培养的和急性分离的CG神经元Ipeak/Cm的IC50分别为0.032mg/L和1.4mg/L. 结论 Eph/Ephrin-Bl信号町影响CG神经元α7-nAChRs的电流,提示Eph/Ephrin-Bl可能参与交感神经系统功能的调控.

EFFECT OF EphB/Ephrin-B1 SIGNAL ON THE CURRENTS OF α7-nAChRs ON CILIARY GANGLION NEURONS

Objective Recent researches showed that Eph/Ephrin tyrosine kinase family plays an important role in the development and functional maintanence of nervous system, but it is unclear if it affects sympathetic nervous system.The study is to detect the effect of EphB/Ephrin-B1 signal on the currents of nicotinic acetylcholine receptor (α7-nAChRs) on ciliary ganglion neurons. Methods Currents of α7-nAChRs on cultured and acute-associated ciliary ganglion neurons were recorded respectively. Cells were randomly divided into four groups: the control group, the Ephrin B1Fc treated group (cells stimulated with different concentrations of Ephrin-B1Fc which is a recombination of IgG and Ephrin-B1), IgG treated group (cells stimulated with IgG at the same concentration of IgG needed for Ephrin-B1Fc recombination), Ephrin-B1 treated group (cells stimulated with Ephrin-B1 at the same concentration of Ephrin-B1 needed for Ephrin-B1Fc recombination). Results There was no difference between the control, IgG and Ephrin-B1 groups, but Ephrin-B1Fc could inhibit α7-nAChRs currents of both cultured and acute-associated CG neurons in a concentration-dependent manner. The inhibition happened quickly and was reversible. ICSUB>50/SUB> of ISUB>peak/SUB>/CSUB>m/SUB> of cultured and acute associated cells was 0.032mg/L and 1.4mg/L respectively. Conclusion The results showed that EphB/Ephrin-B1 signal can affect currents of CG α7-nAChRs. Is suggests that