甘露醇对兔肾血流量及一氧化氮的影响

观察不同剂量甘露醇对兔肾脏功能，肾血流量及一氧化氮（NO）生成的影响，探讨甘露醇诱导急性肾功能衰竭（ARF）的可能机制，18只健康新西兰大白兔随机分为三组，分别给予小剂量甘露醇，生理盐水，大剂量甘露醇并自由饮水，观察肾脏功能，肾血流量及血和尿中NO代谢终产物NO2^-/NO3^-的变化，小剂量甘露醇组肾功能，肾血流量及血NO2^-/NO3^-与对照组比较差异均不显著（P＞0．05），而大剂量并自由饮水组肾功能，肾血流量明显下降，血NO2^-/NO3^-明显上升，与对照组及小剂量组比较差异有显著性（P＞0．05）。三组间尿NO2^-/ NO3^-排泄速率差异无显著性（P＞0．05），提示大剂量甘露醇诱导急性肾功能衰竭与其明显降低肾血流量有关，而内皮衍内舒张因子（EDRF）生成似有代偿性增高。

Changes of renal blood flow and serum NO2-/NO3- in rabbits treated with mannitol at different dosages]

To observe the changes of renal blood flow and serum NO2-/NO3- in rabbits administrated with mannitol at different dosages, and investigate the mechanism of acute renal failure (ARF) induced by massive mannitol. Eighteen healthy male Zealand rabbits were randomly divided into 3 groups: minor mannitol group(n = 6, 8 g.kg-1 within 2 hours), control group (n = 6, saline of the same volume), massive mannitol group with free water taking(n = 6, mannitol 40-60 g.kg-1 with 3 days). The changes of renal function, renal blood flow and NO2-/NO3- in serum and urine were observed. The results showed that: no significant change in creatinine clearance (Ccr), renal blood flow (RBF) and serum NO2-/NO3- was found between minor mannitol group and control group. In massive mannitol group with free water taking, Ccr and RBF significantly decreased and serum NO2-/NO3- significantly increased (P > 0.05). The results suggest that ARF induced by massive mannitol is associated with a significant decline of RBF, on the other hand, endothelium-derived relaxing factor(EDRF) maybe compensatively increase.