新生鼠呼吸道感染CVB3致气道炎症的病理学观察

目的：观察病毒感染对气道炎症、肺炎形态学的影响以及持续的气道反应性增高，探讨病毒感染与呼喘的关系。方法：建立新生儿大鼠病毒感染模型，出生5d新生鼠超声雾化吸入柯萨奇病毒B3（CVB3），接种后10d取血查CVB3－IgM；接种后10d,30d分别观察湿肺质量与鼠体质量比值（LW／BW）、气道肺病理学改变。结果：病毒组新生鼠血清中CVB3－IgM的D值均在对照组-/x 2s以上接种后10dLW/BW比值病毒组明显高于对照组（P＜0．01）。病理学检查：急性期炎症改变显；接种后30d仍有持续的形态学及细胞学改变。结论：新生鼠吸入CVB3模型成立，并有持续的气道炎症及肺形态学改变。

Pathological observation of airway inflammation after neonatal CVB3 inoculation in rats

Objective: To observe the effect of coxsackie virus B3 on airway tract and lung morphology, and to study the relation between CVB infection and asthma. Methods: We established CVB3 infective model: 5 d neonatal rats inhaled CVB3 by ultrasonic brume. CVB3-IgM was examined 10 d after inoculating of CVB3, and LW/BW, airway tract and lung pathological change 10 d and 30 d after inoculation of CVB3 were observed. Results: Rats from the virus group had higher D of CVB3-IgM than control's (+2s ) and had higher LW/BW 10 d after inoculation of CVB3 than control (P<0.01). Neonatal rats had acute inflammatory changes 10 d after inoculation of CVB3 and persistent changes in morphology and cytology. Conclusion: Neonatal rats virus model is established. Respiratory infection by CVB3 in neonatal rats has persistent changes in airway tract inflammatory and morphology.