A continuous T-cell line from a patient with Sézary syndrome |
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Authors: | K. Kaltoft S. Bisballe H. Fogh Rasmussen K. Thestrup-Pedersen K. Thomsen W. Sterry |
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Affiliation: | (1) Department of Dermatology, Institute of Human Genetics, Aarhus, Denmark;(2) Department of Dermatology, University of Aarhus, Aarhus, Denmark;(3) Department of Dermatology, The Finsen Institute, Copenhagen, Denmark;(4) Department of Dermatology, University of Kiel, Federal Republic of Germany |
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Abstract: | Summary A continuous cell line, Se-Ax, from a patient with Sézary syndrome has been established. The Se-Ax cell line is IL-2 dependent, requires human serum for permanent growth, and has the following phenotype: CD1-, CD2+, CD3+, CD4-, CD5-, CD8-, CD20-, CD25+; it expresses the T9, T10, and HLA-DR antigens. This cell line reveals multiple chromosome aberrations as seen in the most abundant abnormal clone in peripheral blood. Therefore, it is not unlikely that it derives from tumor cells. A putative cytotoxic cell line derived from the same patient has only weak killer-cell activity against the autologous permanent cell line: this CD8+ cytotoxic cell line has a 14q+ chromosomal marker. The fact that the patient demonstrated no natural killer-cell or activated killer-cell activity against the Se-Ax cell line may in part explain the successful establishment of the continuous cell line from bulk culture. |
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Keywords: | T cells Sé zary syndrome Natural killer cells Chromosome aberrations |
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