己酮可可碱对非酒精性脂肪性肝炎大鼠肝NF-κB活性作用的研究

目的探讨己酮可可碱(PTX)对非酒精性脂肪性肝炎(NASH)大鼠肝核因子-κB(NF-κB)蛋白的活性作用。方法SD大鼠40只,标准饲料喂养1周后,随机分为4组:对照组、12周模型组、16周模型组和PTX治疗组。用高脂饮食法建立大鼠非酒精性脂肪性肝炎模型,采用免疫组织化学染色法检测大鼠肝组织NF-κB和κB抑制蛋白α(IκB-α)的表达,应用Western blotting的方法检测肝组织中NF-κB的表达。结果免疫组织化学及Westernblotting结果表明,与对照组相比,模型组及PTX治疗组大鼠肝NF-κB的表达显著增加(P<0.05),IκB-α显著降低(P<0.05)。与16周模型组相比,PTX治疗组NF-κB的表达有所降低,IκB-α有显著提高(P<0.05)。结论PTX能抑制大鼠肝IκB的降解,减少NF-κB的活化。

THE EFFECT OF PENTOXIFYLLINE ON HEPATIC NF-κB ACTIVITY OF NONALCOHOLIC STEATOHEPATITIS IN RATS

Objective To investigate the effect of pentoxifylline on hepatic NF-κB activity of nonalcoholic steatohepatitis(NASH) in rats. Methods Rats fed with a standard diet for one week were randomly divided into four groups： the control group(n=10), the 12 week model group(n=10), the 16 week model group(n=10) and the PTX treatment group(n=10). Two model groups and the PTX treatment group were fed with a high fat diet and the control group with a standard diet.After 12 week, the 12 week model group were sacrificed and the treatment group were given PTX ［16mg/(kgI>&#/I>8226;d)］ by intragastric administration. Then all the remaining rats were sacrificed at the end of the 16th week. The expressions of NF-κB and inhibitor κB-α(IκB-α) proteins in livers were analyzed by immunohistochemistry. The expression of NF-κBp65 protein was determined by Western blotting. Results The expression of NF-κBp65 increased significantly in the model groups and the PTX treatment group (vs the control group, P<0.05) but decreased in the PTX treatment group (vs the model groups);