抑制STAT3基因表达对宫颈癌SiHa细胞放射敏感性的影响

Inhibition of STAT3 enhances radiosensitivity of human cervical carcinoma cell line SiHa

Objective To study the effect of silencing STAT3 expression by RNA interference on radiosensitivity of human cervical carcinoma SiHa cells. Methods Vectors containing short hairpin RNA （shRNA） targeting STAT3 （pSTAT3 -siRNA） were constructed and transfected into SiHa cells. The mRNA and protein expressions of STAT3 were determined by RT - PCR and Western blotting, respectively. The different group cells were irradiated by different doses of X - rays （0, 2, 4, 6, and 8 Gy） via the 6MV lin- earity accelerator. The apoptotic rates were detected by flow cytometry and the changes in radiosensitivity were determined by colony formation test. Results The STAT3 expression in pSTAT3 - siRNA group was significantly inhibited, compared with other groups. The apoptotic rate in pSTAT3 - siRNA group was significantly higher than that in other groups. The SF2 values in pSTAT3 - siRNA group were lower than those in other groups. Conclusion The STST3 specific shRNA expression vector effectively suppresses the ex- pression of STAT3 gene in SiHa cells and enhances their radiosensitivity.