| 斑点杂交法检测SEN病毒感染 |
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| 引用本文： | 杨群,田德英,许东,葛娅,任星峰,宋佩辉.斑点杂交法检测SEN病毒感染[J].第四军医大学学报,2004,25(20):1877-1879. |
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| 作者姓名： | 杨群 田德英 许东 葛娅 任星峰 宋佩辉 |
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| 作者单位： | 广州军区武汉总医院传染科,湖北,武汉,430070;华中科技大学同济医学院附属同济医院传染科,湖北,武汉,430030 |
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| 摘 要： | 目的:探讨斑点杂交法检测SEN病毒(SENV)感染的应用价值.方法:应用地高辛标记、制备SENV部分基因探针并用斑点杂交技术检测SENV DNA,与聚合酶链反应(PCR)检测结果相比较. 结果: 在191份血清中,采用斑点杂交法检出6份阳性,检出率为3.1%. PCR方法检测出11份阳性,阳性率5.8%. 结论: 制备的地高辛探针具有SENV特异性,以地高辛标记的SENV探针可用于检测SENV感染.
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| 关 键 词： | SEN病毒 斑点杂交 聚合酶链反应 |
| 文章编号： | 1000-2790(2004)20-1877-03 |
| 修稿时间： | 2004年5月8日 |
Detection of SENV DNA in sera by dot blot |
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| YANG Qun ,TIAN De-Ying ,XU Dong ,GE Ya ,REN Xing-Feng ,SONG Pei-Hui.Detection of SENV DNA in sera by dot blot[J].Journal of the Fourth Military Medical University,2004,25(20):1877-1879. |
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| Authors: | YANG Qun TIAN De-Ying XU Dong GE Ya REN Xing-Feng SONG Pei-Hui |
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| Institution: | YANG Qun 1,TIAN De-Ying 2,XU Dong 2,GE Ya 1,REN Xing-Feng 1,SONG Pei-Hui 2 1Department of Infectious Diseases,Wuhan General Hospital,Guangzhou Command,Wuhan 430070,China,2Department of Infectious Diseases,Tongji Hospital,Tongji Medical College,Huazhong University of Science & Technology,Wuhan 430030,China |
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| Abstract: | AIM: To explore the availability of dot-blot assay in the detection of SEN virus infection. METHODS: Nuclear acid was extracted from serum specimens, and dot-blot analysis and polymerase chain reaction(PCR) were carried out respectively to detect SENV DNA. RESULTS: Among 191 samples, 6 were found positive by dot-blot, and 11 were found positive by PCR. CONCLUSION: The probe prepared by us is SENV-specific. Dot-blot hybridization with DIG-labeled SENV DNA probe can be used to detect SENV DNA in sera samples. |
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| Keywords: | SEN virus dot-blot polymerase chain reaction |
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