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四川荥经县艾伯特埃希菌的分离与鉴定
引用本文:杨德举,王红,谢姝,李展翅,张玲,闫国栋,杨红梅,林雨巍,高霞,姜航星,柴良玉,赵艺,李群. 四川荥经县艾伯特埃希菌的分离与鉴定[J]. 中国热带医学, 2018, 18(12): 1187-1190. DOI: 10.13604/j.cnki.46-1064/r.2018.12.03
作者姓名:杨德举  王红  谢姝  李展翅  张玲  闫国栋  杨红梅  林雨巍  高霞  姜航星  柴良玉  赵艺  李群
作者单位:1. 四川省荥经县疾病预防控制中心,四川 荥经 625200;
2. 四川省自贡市疾病预防控制中心,四川 自贡 643000
基金项目:四川省卫生和计划生育委员会科研课题(No.150259); 自贡市重点科技计划项目(No.2013S06); 传染病预防控制国家重点实验室开放课题(No.2016SKLID309)
摘    要:目的 按照自贡市疾病预防控制中心编制的艾伯特埃希菌的检测程序,对四川荥经县采集的鸡肠样品中艾伯特埃希菌的携带情况进行检测,了解该地区家禽中艾伯特埃希菌的携带情况及菌株特征。方法 采集荥经县农贸市场、棒棒鸡店铺、活鸡宰杀点的新鲜鸡肠样品163份,按照艾伯特埃希菌的检测程序对样品进行检测,鸡肠内容物经EC肉汤增菌后,PCR检测eae基因,eae阳性样品的增菌液接种于麦康凯琼脂,37 ℃,24 h培养后挑取不发酵乳糖、eae阳性的疑似菌株,使用多重PCR检测及多位点序列分析(MLST)对疑似菌株进行鉴定。结果 自贡市疾病预防控制中心编制的艾伯特埃希菌的检测程序可行有效,在荥经县共采集了新鲜的鸡肠样163份品,从3份样品中分离到3株疑似艾伯特埃希菌菌株,经多重PCR检测及多位点序列分析(MLST)鉴定,3株疑似菌株均为艾伯特埃希菌。本次采集的样品艾伯特埃希菌的检出率为1.84%(3/163)。结论 四川荥经县地区鸡肠中存在艾伯特埃希菌,分离到的菌株丰富了艾伯特埃希菌生物学特征、流行病特征研究的菌株数据库。

关 键 词:艾伯特埃希菌  eae基因  多重PCR  多位点序列分型  
收稿时间:2018-08-13

Isolation and identification of Escherichia albertii in Yingjing,Sichuan
YANG Deju,WANG Hong,XIE Shu,LI Zhanchi,ZHANG Ling,YAN Guodong,YANG Hongmei,LIN Yuwei,GAO Xia,JIANG Hangxing,CHAI Liangyu,ZHAO Yi,LI Qun. Isolation and identification of Escherichia albertii in Yingjing,Sichuan[J]. China Tropical Medicine, 2018, 18(12): 1187-1190. DOI: 10.13604/j.cnki.46-1064/r.2018.12.03
Authors:YANG Deju  WANG Hong  XIE Shu  LI Zhanchi  ZHANG Ling  YAN Guodong  YANG Hongmei  LIN Yuwei  GAO Xia  JIANG Hangxing  CHAI Liangyu  ZHAO Yi  LI Qun
Affiliation:1.Yingjing Center for Disease Control and Prevention, Yingjing, Sichuan 625200, China
Abstract:Objective In order to investigate the prevalence and characteristics of Escherichia albertii in Yingjing, we detected the Escherichia albertii carrying status of the chicken intestine samples collected from Yingjing Sichuan Province according to the detection procedure of Escherichia albertii edited by Zigong Center of Disease Control and Prevention (Zigong CDC). Methods A total of 163 samples of chicken intestines which were collected from open market of agricultural products, Bangbang chicken shop, slaughtering location of chicken and so on, were detected according to the detection procedure. The chicken intestines were enriched with EC Medium (Escherichia coli Medium), the positive PCR detection of eae gene for chicken intestines were inoculated in MacConkey Sorbitol Agar (MSA) for 24 hours on 37 °C. The eae-positive lactose non-fermenting isolates further classified as Escherichia albertii by multiplex PCR and multilocus sequence typing (MLST). Results The detection procedure edited by Zigong CDC was feasible and effective. One hundred and sixty-three chicken intestines samples were collected in this study, three suspected strains were detected from three samples, and which were classified as Escherichia albertii by multiplex PCR and MLST. The detection rate was 1.84%(3/163). Conclusions This study indicate that Escherichia albertii is existed in Yingjing, and the strains isolated in this study enrich the strain database of biological characteristics and epidemiological studies of Escherichia albertii.
Keywords:Escherichia albertii   eae gene  multiplex PCR  MLST  
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