外周血Th1、Th17细胞比值与布氏杆菌性脊柱炎术后复发的相关性

目的 分析外周血Th1、Th17细胞表达与布氏杆菌性脊柱炎术后复发的相关性.方法 选取2017年1月—2020年3月河北北方学院附属第一医院骨外科收治布氏杆菌性脊柱炎患者68例作为研究对象(脊柱炎组),选取同期医院健康体检者70例为健康对照组.检测2组外周血Th1、Th17细胞比例,血清白介素-2(IL-2)、干扰素-...

Correlation between the ratio of Th1 and Th17 cells in peripheral blood and the recurrence of brucellosis spondylitis

Objective To analyze the correlation between the ratio of Th1 and Th17 cells in peripheral blood and the recurrence of brucellosis spondylitis.Methods From January 2017 to March 2020,68 patients with brucellosis spondylitis admitted to the Department of Orthopedic Surgery of the First Affiliated Hospital of Hebei North University were selected as the research object(spondylitis group),and 70 patients with healthy physical examination in the hospital during the same period were selected as the healthy control group.The proportion of Th1 and Th17 cells in the peripheral blood of the two groups was detected,and the levels of serum interleukin 2(IL-2),interferon gamma(IFN-γ),IL-17,IL-23,and IL-6 were detected.To analyze the risk factors affecting the recurrence of brucellosis spondylitis after surgery.Results Compared with the healthy control group,the proportion of peripheral blood mononuclear cells Th17 cells in the spondylitis group and the preoperative serum IL-17,IL-6,IL-23 levels increased(t/P=5.711/0.000,45.567/0.000,58.709/0.000,17.221/0.000),the proportion of peripheral blood mononuclear cells Th1 cells and serum IL-2,IFN-γlevels decreased(t/P=34.024/0.000,8.991/0.000,5.948/0.000).Compared with the non-recurring subgroup,the proportion of peripheral blood mononuclear cells Th17 cells and postoperative serum IL-17,IL-6,IL-23 levels in the recurring subgroup increased(t/P=5.509/0.000,37.286/0.000,46.074/0.000,18.982/0.000),the proportion of peripheral blood mononuclear cells Th1 cells and serum IL-2,IFN-γlevels decreased(t/P=15.960/0.000,3.450/0.001,2.712/0.000).The percentage of Th1,Th17 cells and IL-17,IL-6,IL-23,IL-2,IFN-γafter surgery are all risk factors for the recurrence of brucellosis spondylitis[OR(95%CI)=2.375(1.657~3.405),2.454(1.737~3.472),2.273(1.661~3.109),2.219(1.619~3.043),2.278(1.684~3.082),2.261(1.546~3.308),2.337(1.624~3.364)].Conclusion The proportion of Th17 cells in the peripheral blood of patients with recurrence after brucellosis spondylitis increases,while the proportion of Th1 cells decreases.The expression of Th1 and Th17 cells is closely related to the recovery of brucellosis spondylitis after surgery.