荧光定量PCR对1501例NGU患者病原体基因检测结果分析

目的用实时荧光定量聚合酶链反应（FQ-PCR）技术准确度定量检测江门市区凝有非淋菌性尿道炎患者沙眼衣原体（CT）、解脲支原体（UU）基因,进一步了解UU、CT的感染情况,为临床治疗提供依据。方法运用实时荧光定量PCR法（FQ-PCR）对1501例凝有非淋菌性尿道炎患者同时进行CT、UU检测。结果CT的阳性率是13.1%,UU的阳性率是33.9%,二者之间阳性率比较有显著性差异。结论1.江门市区非淋菌性尿道炎患者病原体CT、UU中占有一定比例,二者之间感染率比较有显著性差异。2.荧光实时定量PCR检测UU、CT具有操作简单、反应时间短、重复性好、结果客观准确、敏感性和特异性好等优点,其结果对临床诊断、治疗有一定指导意义。

The detection and analysis of the gene of causative agents of 1501 cases of non——gonococcal urethritis by fluorescence quantitative PCR

Objective:To detect and analysis chlamydia trachomatis and ureaplasm urealyticum infection in non-gonococcal urethritis by fluorescence quantitative PCR assay in Jiangmen city.Methods: The chlamydia trachomatis and ureaplasm urealyticum of non-gonococcal urethritis by fluorescence quantitative PCR assay.Results: The positive rete of CT were 13.1%,and the positive rete of UU were 33.9%,The positive rete were respective.The mixed infect were rate in the group.Conclusion: 1.CT and UU infection play a certain role in non-gonococcal urethritis and physician should pay attention for it.2.The fluorescence quantitative PCR assays showed advantages which were mainpulated simply,time-saving,more extra,more sensitive and more spectific in detecting CT and UU.FQ-PCR had directive meaning for dagnosis and treatmeat.