| HPLC法测定乳疮丸中绿原酸的含量 |
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| 引用本文： | 杨静,高文远.HPLC法测定乳疮丸中绿原酸的含量[J].天津药学,2010,22(3):16-18. |
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| 作者姓名： | 杨静 高文远 |
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| 作者单位： | 1. 天津大学药学院,天津,300072;天津渤海职业技术学院,天津,300402
2. 天津大学药学院,天津,300072 |
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| 摘 要： | 目的：建立HPLC法测定乳疮丸中绿原酸含量的方法。方法：色谱柱为YMC-Pack ODS-A（250mm×4.6mm,5μm）;流动相为乙腈-水-磷酸（10∶90∶0.4）;检测波长为327nm;流速为1.0ml/min;柱温为30℃;进样量10μl。结果：绿原酸测定的线性范围为0.0308~0.308μg,回归方程为Y=98466.04X-24944.48,r=0.999961,平均回收率为100.55%,RSD为1.06%（n=6）。结论：该方法结果准确,操作简单,重现性好,可作为乳疮丸质量控制方法。
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| 关 键 词： | 乳疮丸 高效液相色谱法 绿原酸 |
Determination of chlorgenic acid in Ruchuang pill by HPLC |
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| Yang Jing,Gao Wenyuan.Determination of chlorgenic acid in Ruchuang pill by HPLC[J].Tianjin Pharmacy,2010,22(3):16-18. |
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| Authors: | Yang Jing Gao Wenyuan |
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| Institution: | 1.Tianjin Bohai Vocational Technical College,Tianjin 300042;2.College of Pharmaceutical Science and Technology Tianjin University,Tianjin 300072) |
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| Abstract: | Objective：To establish an HPLC method for the determination of chlorgenic acid in Ruchuang Pill.Methods：The YMC-Pack ODS-A column（250 mm×4.6 mm,5 μm）was used,and the mobile phase consisted of acetonitrile-water-phosphate（10∶90∶0.4）,the flow rate of which was 1.0 ml/min.The column temperature was 30 ℃,and the dectection wavelength was at 327 nm.Results：The cablibration curve was linear within a range of 0.030 8～0.308 μg,the regression equation of which was Y=98 466.04 X-24 944.48,r=0.999 961,and average recovery was 100.55% with RSD of 1.06.Conclusions：The method is simple and repeatable and can be applied to the quality control of Ruchuang Pill. |
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| Keywords: | Ruchuang pill HPLC chlorgenic acid determination |
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