茶多酚对移植性小鼠肿瘤微血管密度影响研究

目的:应用免疫组化法,观察茶多酚口服、静脉与局部注射3种给药途径对肿瘤微血管密度(MVD)的影响,以判定茶多酚是否具有抗肿瘤新生血管形成效应.方法:移植性S180小鼠肿瘤动物模型建立后,分别按茶多酚口服、局部注射、腹腔注射及人参皂苷Rg3口服、CTX腹腔注射给药,5天后取肿瘤组织,常规石腊包埋、切片,应用免疫组化法观察肿瘤组织MVD.结果:茶多酚口服、局部注射可明显减少S180荷瘤小鼠MVD,而腹腔注射则无明显效果.结论:茶多酚具有抗肿瘤新生血管形成作用.

Effect of Tea Polyphenols on Density of Tumor Blood Capillary of Transplantation Mice

Objective:To apply immunohistochemical method to observe the effect of tea polyphenols in oral administration,intravenous injection and local injection on the microvessel density(MVD),in order to determine whether tea polyphenols have the function of preventing neoplasm of tumor blood vessels.Methods:Transplatation S_(180) mice tumor models were given tea polyphenols by oral administration,local injection and intraperitoneal injection,and were given panaxsaponin Rg_3 by oral administration and intraperitoneal injection.Five days later,the tumor tissue was embedded,cut into slices and examined with immunohistochemical method for MVD.Results:Oral administration and local injection of tea polyphenols could markedly reduce the density of tumor blood vessels,while intraperitoneal injection did not produce any effect.Conclusion:Tea polyphenols have the effect of preventing neoplasm of tumor blood vessels.