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Quantitation of immunoglobulin A in the human chorioamniotic membrane |
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| Authors: | A T Berezowski S P Cunha J C Costa R R Santos G Duarte |
| Affiliation: | 1. Department of Gynecology and Obstetrics, Faculty of Medicine of Botucatu, State University of Sã Paulo, Botucatu, Brazil;2. Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Riberão Preto, São Paulo, Brazil;3. Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil;4. Department of Parasitology, Microbiology and Immunology, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil |
| Abstract: | Thirty-eight chorioamniotic membranes were obtained from normal patients divided into three groups according to time of rupture. A protein extract was obtained by destruction and differential centrifugation and immunoglobulin A was measured by the method of Mancini. The authors demonstrated that it is possible to quantitate immunoglobulin A. No statistically significant differences were observed between groups. |
| Keywords: | Immunoglobulin A Chorioamniotic membranes Amniotic fluid Normal patients Time of rupture Protein extract obtained Destruction and differential centrifugation Mancini method |
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