| 葎草酮抑制人胃癌细胞SGC-7901 N-乙酰基转移酶1活性的酶动力学研究 |
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| 引用本文： | 高世勇,郎朗,邹翔,汲晨锋,季宇彬,马强,岳磊,曲中原,尚明.葎草酮抑制人胃癌细胞SGC-7901 N-乙酰基转移酶1活性的酶动力学研究[J].中草药,2010,41(6):931-934. |
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| 作者姓名： | 高世勇 郎朗 邹翔 汲晨锋 季宇彬 马强 岳磊 曲中原 尚明 |
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| 作者单位： | 哈尔滨商业大学生命科学与环境科学研究中心,药物研究所博士后科研流动站,黑龙江,哈尔滨,150076;国家教育部抗肿瘤天然药物工程研究中心,黑龙江,哈尔滨,150076 |
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| 基金项目： | 黑龙江省教育厅重点项目 |
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| 摘 要： | 目的探讨葎草酮对人胃癌SGC-7901细胞N-乙酰基转移酶1(NAT1)酶动力学常数的影响。方法采用HPLC法,以NAT1酶特异性底物对氨基苯甲酸(PABA)为底物,以SGC-7901完整细胞及细胞质内PABA被NAT1乙酰化为Ac-PABA的速度为NAT1酶的反应速率,采用双倒数作图法,以底物PABA浓度的倒数对NAT1反应速率的倒数进行直线回归,得出回归方程,计算米氏常数(Km)和最大反应速率(Vmax)。结果酶动力学研究表明,以PABA为底物,对于SGC-7901完整细胞,阴性对照组的Km和Vmax分别为(3.910±0.087)μmol/L、(0.306 0±0.006 7)pmol(1×106个细胞),葎草酮组的Km和Vmax分别为(3.830±0.123)μmol/L、(0.275 0±0.005 8)pmol(1×106个细胞),对于SGC-7901细胞质,阴性对照组的Km和Vmax分别为(760.2±210.2)μmol/L、(0.191 0±0.043 7)pmol/(mg.min),葎草酮组的Km和Vmax分别为(449.0±72.9)μmol/L和(0.094 0±0.010 4)pmol/(mg.min),数据经统计学处理表明对SGC-7901完整细胞或细胞质中的NAT1酶,阴性对照组和葎草酮组的Km没有统计学差异,而Vmax有显著差异。结论葎草酮是SGC-7901人胃癌细胞NAT1酶PABA底物的非竞争性抑制剂。
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| 关 键 词： | 葎草酮 人胃癌细胞SGC-7901 N-乙酰基转移酶1(NAT1) 米氏常数Km 最大反应速率Vmax |
| 收稿时间： | 2010/1/18 0:00:00 |
Effects of humulon on kinetic constants of NAT1 in SGC-7901 cells |
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| GAO Shi-yong,LANG Lang,ZOU Xiang,JI Chen-feng,JI Yu-bin,MA Qiang,YUE Lei,QU Zhong-yuan and SHANG Ming.Effects of humulon on kinetic constants of NAT1 in SGC-7901 cells[J].Chinese Traditional and Herbal Drugs,2010,41(6):931-934. |
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| Authors: | GAO Shi-yong LANG Lang ZOU Xiang JI Chen-feng JI Yu-bin MA Qiang YUE Lei QU Zhong-yuan and SHANG Ming |
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| Institution: | Post-doctoral Research Station, Institute of Materia Medica, Center of Research and Development on Life Sciences and Environment Sciences of Harbin University of Commerce, Harbin 150076, China, 2. MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China;Post-doctoral Research Station, Institute of Materia Medica, Center of Research and Development on Life Sciences and Environment Sciences of Harbin University of Commerce, Harbin 150076, China, 2. MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China;Post-doctoral Research Station, Institute of Materia Medica, Center of Research and Development on Life Sciences and Environment Sciences of Harbin University of Commerce, Harbin 150076, China, 2. MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China;Post-doctoral Research Station, Institute of Materia Medica, Center of Research and Development on Life Sciences and Environment Sciences of Harbin University of Commerce, Harbin 150076, China, 2. MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China;Post-doctoral Research Station, Institute of Materia Medica, Center of Research and Development on Life Sciences and Environment Sciences of Harbin University of Commerce, Harbin 150076, China, 2. MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China;Post-doctoral Research Station, Institute of Materia Medica, Center of Research and Development on Life Sciences and Environment Sciences of Harbin University of Commerce, Harbin 150076, China, 2. MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China;Post-doctoral Research Station, Institute of Materia Medica, Center of Research and Development on Life Sciences and Environment Sciences of Harbin University of Commerce, Harbin 150076, China, 2. MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China;Post-doctoral Research Station, Institute of Materia Medica, Center of Research and Development on Life Sciences and Environment Sciences of Harbin University of Commerce, Harbin 150076, China, 2. MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China;Post-doctoral Research Station, Institute of Materia Medica, Center of Research and Development on Life Sciences and Environment Sciences of Harbin University of Commerce, Harbin 150076, China, 2. MOE Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce, Harbin 150076, China |
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| Abstract: | |
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| Keywords: | humulon human gastric cancer SGC-7901 cells N-acetyltransferase-1(NAT1) Michaelis constant(Km) maximum reaction velocity(Vmax) |
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